Skip to main content
Addgene

We narrowed to 8,865 results for: BLI;

Showing: 3551 - 3600 of 8865 results
  1. KBB109

    Plasmid
    #185066
    Purpose
    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction site
    Depositor
    Insert
    NUP1
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Nup1 C-terminal region, truncated at NsiI restric…
    Promoter
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pLZRS-TrkC-mCherry

    Plasmid
    #182949
    Purpose
    To express the rat TrkC fused in the C-terminus with mCherry
    Depositor
    Insert
    TRk-C-mCherry (Ntrk3 Synthetic, Rat)
    Use
    Retroviral and Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pJA12 Mamu-A1*001:01

    Plasmid
    #180442
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    Mamu-A1*001:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pMOD4-mT2A-mutated_rpsL-BSD-F

    Plasmid
    #182338
    Purpose
    Template plasmid for PCR amplification of initial recombineering cassette
    Depositor
    Insert
    BSD
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pmr101A-cPr-mNeonGreen (ErmR)

    Plasmid
    #177208
    Purpose
    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
    Depositor
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Yeast codon optimized
    Promoter
    Synthetic constitutive promoter
    Available since
    March 16, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pJA13 Mamu-A1*002:01

    Plasmid
    #180443
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    Mamu-A1*002:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pTCF122 Mamu-B*008:01

    Plasmid
    #180455
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    Mamu-B*008:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pTCF194 Mamu-E*02:04

    Plasmid
    #180461
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    Insert
    Mamu-E*02:04
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. SYKA-c014

    Plasmid
    #175488
    Purpose
    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
    Depositor
    Insert
    SYK (SYK Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pLV V5-Senp2

    Plasmid
    #175118
    Purpose
    Lentiviral expression of V5-tagged mouse Senp2
    Depositor
    Insert
    Senp2 (Senp2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. RAB5AA-c006

    Plasmid
    #175476
    Purpose
    Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.
    Depositor
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. MSNA-c007

    Plasmid
    #175467
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-D298. Used for NMR studies.
    Depositor
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir

    Plasmid
    #178046
    Purpose
    CAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Insert
    Renilla_713 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir

    Plasmid
    #178045
    Purpose
    CAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)
    Depositor
    Insert
    Luciferase_1309 shRNAmir
    Use
    Cre/Lox and RNAi; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. pKSB- attP 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174534
    Purpose
    attP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Insert
    attP and 3xP3-GFP-SV40 BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    3xP3
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pKSB- PUb-GFPsv40 GGGG AAGA

    Plasmid
    #174536
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Insert
    PUb-GFP-SV40 BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pKSB- PUb-GFPTub56D GGGG AAGA

    Plasmid
    #174537
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminator
    Depositor
    Insert
    PUb-GFP-Tub56Dterminator BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pISV-DsRed1-nopP

    Plasmid
    #171025
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
    Insert
    nopP
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2 X EN 35S
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. PB CAG-sfGFP-GSAx9-iCre-ERT2

    Plasmid
    #137857
    Purpose
    CAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposon
    Depositor
    Insert
    sfGFP-GSAx9-iCre-ERT2
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  22. rox Neo* rox

    Plasmid
    #134322
    Purpose
    rox-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Insert
    Neo*
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
  23. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  24. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  25. pX335 Mouse 5' Srcap gRNA B

    Plasmid
    #127905
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  26. pX335 Mouse 5' Srcap gRNA A

    Plasmid
    #127904
    Purpose
    Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene
    Depositor
    Insert
    Srcap gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  27. pX330 Mouse 3' Ubf gRNA

    Plasmid
    #127902
    Purpose
    WT Cas9 Vector targeting the 3' end of the mouse Ubf gene
    Depositor
    Insert
    gRNA for Mouse 3' Ubf
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  28. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Insert
    gRNA against human TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  29. pZDonor-U6-myogenin stop codon gRNA

    Plasmid
    #69549
    Purpose
    Expresses guide RNA targeting near the stop codon of myogenin in the mouse genome.
    Depositor
    Insert
    Myogenin C terminus-targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  30. CMV-Lin28a-as

    Plasmid
    #120528
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  31. Fly 2.0 pIDTBlue

    Plasmid
    #117418
    Purpose
    Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control
    Depositor
    Insert
    FLY 2.0
    Use
    Tags
    NA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  32. CAG-EGFP-Lin28IR

    Plasmid
    #120517
    Purpose
    dsRNA expression
    Depositor
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  33. U6Lin28a-as

    Plasmid
    #120536
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  34. U6-Lin28a-s

    Plasmid
    #120535
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  35. CMV-Lin28a-s

    Plasmid
    #120527
    Purpose
    ssRNA expression for dsRNA production
    Depositor
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  36. CMV-Lin28aIR

    Plasmid
    #120524
    Purpose
    dsRNA expression
    Depositor
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
  37. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  38. pJR288

    Plasmid
    #78550
    Purpose
    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    Jan. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  39. pMP71-HLA-B15-His

    Plasmid
    #104848
    Purpose
    HLA-B150101 cloned as a His tag
    Depositor
    Insert
    major histocompatibility complex, class I, B (HLA-B Human)
    Use
    Retroviral
    Tags
    his
    Expression
    Mammalian
    Mutation
    2 differences compared to original B15: L2->R …
    Promoter
    LTR
    Available since
    Dec. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  40. pJL16

    Plasmid
    #117425
    Purpose
    Expression of ARF3
    Depositor
    Insert
    ARF3 (ARF3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  41. pmSod1-Q42L-N’His

    Plasmid
    #117708
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  42. pmSod1-Q123A-N’His

    Plasmid
    #117709
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  43. pmSod1-D83G-N’His

    Plasmid
    #117702
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    D83G
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  44. pHH0103 PQBP1 WW domain

    Plasmid
    #104287
    Purpose
    Bacterial expression of WW domain from PQBP1
    Insert
    PQBP1 WW domain (PQBP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  45. pHH0103 HLA-DQA2 WW domain

    Plasmid
    #104297
    Purpose
    Bacterial expression of WW domain from HLA-DQA2
    Insert
    HLA-DQA2 WW domain (HLA-DQA2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  46. TAP-Amotl2

    Plasmid
    #112834
    Purpose
    transient/stable overexpression
    Insert
    Amotl2 (Amotl2 Mouse)
    Use
    Tags
    TAP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  47. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  48. pHH0103 HYPC WW domain #2

    Plasmid
    #104232
    Purpose
    Bacterial expression of WW domain #2 from HYPC
    Insert
    HYPC WW domain #2 (PRPF40B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  49. GLT-1b delta 4

    Plasmid
    #98707
    Purpose
    mammalian expression of Glt1b delta 4 mutant
    Depositor
    Insert
    Glt1b (Slc1a2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    missing last 4 amino acids at the C-terminus. I52…
    Promoter
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  50. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 3551 - 3600 of 8865 results