We narrowed to 8,865 results for: BLI;
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Plasmid#185066PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction siteDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 C-terminal region, truncated at NsiI restric…PromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-TrkC-mCherry
Plasmid#182949PurposeTo express the rat TrkC fused in the C-terminus with mCherryDepositorInsertTRk-C-mCherry (Ntrk3 Synthetic, Rat)
UseRetroviral and Synthetic BiologyTagsmCherryExpressionMammalianMutationPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA12 Mamu-A1*001:01
Plasmid#180442PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-A1*001:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD4-mT2A-mutated_rpsL-BSD-F
Plasmid#182338PurposeTemplate plasmid for PCR amplification of initial recombineering cassetteDepositorInsertBSD
UseMouse Targeting; Flp / frtTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mNeonGreen (ErmR)
Plasmid#177208PurposePlasmid with p15A origin for constitutive mNeonGreen expression in E. coliDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationYeast codon optimizedPromoterSynthetic constitutive promoterAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA13 Mamu-A1*002:01
Plasmid#180443PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-A1*002:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF122 Mamu-B*008:01
Plasmid#180455PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-B*008:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF194 Mamu-E*02:04
Plasmid#180461PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-E*02:04
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLV V5-Senp2
Plasmid#175118PurposeLentiviral expression of V5-tagged mouse Senp2DepositorInsertSenp2 (Senp2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAB5AA-c006
Plasmid#175476PurposeProtein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.DepositorInsertRAB5A (RAB5A Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Renilla_shRNAmir
Plasmid#178046PurposeCAG tdGFP Renilla shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertRenilla_713 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-L-bpA-2xSV40pA-L-tdGFP-Luciferase_shRNAmir
Plasmid#178045PurposeCAG tdGFP Luciferase shRNAmir cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)DepositorInsertLuciferase_1309 shRNAmir
UseCre/Lox and RNAi; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RB-PE2
Plasmid#173902PurposeSB-transposon with constitutive expression of SpCas9 PE2DepositorInsertPE2
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- attP 3xP3-GFPsv40 GGGG AAGA
Plasmid#174534PurposeattP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertattP and 3xP3-GFP-SV40 BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-GFPsv40 GGGG AAGA
Plasmid#174536PurposePUb-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-GFP-SV40 BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-GFPTub56D GGGG AAGA
Plasmid#174537PurposePUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminatorDepositorInsertPUb-GFP-Tub56Dterminator BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pISV-DsRed1-nopP
Plasmid#171025PurposeAgrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.DepositorInsertnopP
UseTagsExpressionPlantMutationPromoter2 X EN 35SAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pL18-ccdB
Plasmid#164898PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-sfGFP-GSAx9-iCre-ERT2
Plasmid#137857PurposeCAG-sfGFP-GSAx9-iCre-ERT2 piggyBac transposonDepositorInsertsfGFP-GSAx9-iCre-ERT2
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
rox Neo* rox
Plasmid#134322Purposerox-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Dre/roxTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalphaKanR
Plasmid#160230PurposeLike pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'
Plasmid#91803PurposeExpresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be usedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX335 Mouse 5' Srcap gRNA B
Plasmid#127905PurposeCas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap geneDepositorInsertSrcap gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX335 Mouse 5' Srcap gRNA A
Plasmid#127904PurposeCas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap geneDepositorInsertSrcap gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 Mouse 3' Ubf gRNA
Plasmid#127902PurposeWT Cas9 Vector targeting the 3' end of the mouse Ubf geneDepositorInsertgRNA for Mouse 3' Ubf
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB700-TTN-Puro
Plasmid#128323PurposeSP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)DepositorInsertgRNA against human TTN for activation
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-U6-myogenin stop codon gRNA
Plasmid#69549PurposeExpresses guide RNA targeting near the stop codon of myogenin in the mouse genome.DepositorInsertMyogenin C terminus-targeting guide RNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Lin28a-as
Plasmid#120528PurposessRNA expression for dsRNA productionDepositorInsertLin28 (Lin28a Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fly 2.0 pIDTBlue
Plasmid#117418PurposeDrosophila melanogaster gene cloned into pIDTBlue vector for internal extraction controlDepositorInsertFLY 2.0
UseTagsNAExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Lin28IR
Plasmid#120517PurposedsRNA expressionDepositorInsertLin28 (Lin28a Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6Lin28a-as
Plasmid#120536PurposessRNA expression for dsRNA productionDepositorInsertLin28 (Lin28a Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-Lin28a-s
Plasmid#120535PurposessRNA expression for dsRNA productionDepositorInsertLin28 (Lin28a Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Lin28a-s
Plasmid#120527PurposessRNA expression for dsRNA productionDepositorInsertLin28 (Lin28a Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Lin28aIR
Plasmid#120524PurposedsRNA expressionDepositorInsertLin28 (Lin28a Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9CR5
Plasmid#120227PurposepCas9CR4 with stop codon to prevent translation of ssrA degradation tagDepositorInsertSpCas9
UseSynthetic BiologyTagsExpressionMutationStop codon inserted before ssrA degradation tagPromoterpTetAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJR288
Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-HLA-B15-His
Plasmid#104848PurposeHLA-B150101 cloned as a His tagDepositorInsertmajor histocompatibility complex, class I, B (HLA-B Human)
UseRetroviralTagshisExpressionMammalianMutation2 differences compared to original B15: L2->R …PromoterLTRAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pmSod1-Q42L-N’His
Plasmid#117708Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ42LPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-Q123A-N’His
Plasmid#117709Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ123APromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-D83G-N’His
Plasmid#117702Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD83GPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 PQBP1 WW domain
Plasmid#104287PurposeBacterial expression of WW domain from PQBP1DepositorInsertPQBP1 WW domain (PQBP1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HLA-DQA2 WW domain
Plasmid#104297PurposeBacterial expression of WW domain from HLA-DQA2DepositorInsertHLA-DQA2 WW domain (HLA-DQA2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAP-Amotl2
Plasmid#112834Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsTAPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxEH
Plasmid#104977PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1644 (MXAN_1644 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HYPC WW domain #2
Plasmid#104232PurposeBacterial expression of WW domain #2 from HYPCDepositorInsertHYPC WW domain #2 (PRPF40B Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
GLT-1b delta 4
Plasmid#98707Purposemammalian expression of Glt1b delta 4 mutantDepositorInsertGlt1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationmissing last 4 amino acids at the C-terminus. I52…PromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-C
Plasmid#97029PurposeHis6-MBP-N10-tev-yORFDepositorTypeEmpty backboneUseTagsHis6 tag, MBP domain, N10 linker, and TEV cleavag…ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only