We narrowed to 75,796 results for: Rest

1. iYFP-Parkin Plasmid

   Plasmid

   #197107

   Purpose

   Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

   Depositor

   Dori Woods

   Article

   Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

   Insert

   parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
   

   Use

   Retroviral

   Tags

   YFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 c-SRC (WT)

   Plasmid

   #42202

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   c-SRC (SRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L176F mutation compared to reference sequence NM_…

   Promoter

   CMV

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hPDGFRA-GFP

   Plasmid

   #22919

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-2xFYVE

   Plasmid

   #140050

   Purpose

   Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

   Depositor

   Harald Stenmark

   Article

   Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   two FYVE domains (amino acids 147-223), linked by…

   Promoter

   CMV

   Available since

   May 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKII-Jaws-KGC-GFP-ER2

   Plasmid

   #65015

   Purpose

   AAV-mediated expression of Jaws under the CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-KGC-GFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CaMKII

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.1 SIRT1-FL

   Plasmid

   #105670

   Purpose

   Mammalian expression construct of mouse SIRT1 full length isoform

   Depositor

   Ullas Kolthur-Seetharam

   Article

   Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

   Insert

   SIRT1 (Sirt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous base changes not affecting the protein…

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hsyn-Jaws-GFP-ER2

   Plasmid

   #65016

   Purpose

   AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-GFP-ER2
   

   Use

   AAV

   Tags

   ER2 and GFP

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   human synapsin

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-GFP-IKBalpha-mut (super repressor)

   Plasmid

   #15264

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   IKB alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Serine 32 to Alanine, Serine 36 to Alanine

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. fck-Jaws-GFP-ER2

    Plasmid

    #65013

    Purpose

    Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-GFP-ER2
    

    Use

    Lentiviral

    Tags

    ER2 and GFP

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-noSV40-humanEC1.45

    Plasmid

    #173946

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. fck-Jaws-KGC-GFP-ER2

    Plasmid

    #65012

    Purpose

    Lentivirus-mediated expression of Jaws under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    Lentiviral

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. scAAV-TTR-mFgf15

    Plasmid

    #190594

    Purpose

    AAV construct containing Fgf15 cds under control of TTR promoter

    Depositor

    Roel Nusse

    Article

    Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

    Insert

    Fgf15 (Fgf15 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ttr

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hGRM1-RFP

    Plasmid

    #22920

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. HOXB6 (human) FLAG pMSCV

    Plasmid

    #8534

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB6 (HOXB6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    contains improved ribosomal binding site and FLAG…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pOPINK-ALG13 (OTU, aa 216-353)

    Plasmid

    #61414

    Purpose

    Expresses human ALG13 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-215 and aa 354-1137.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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