162,999 results

1. pPEG

   Plasmid

   #162466

   Purpose

   Entry vector for gRNA insertion in plant prime editing expression vector

   Depositor

   Adam Bogdanove

   Article

   Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

   Type

   Empty backbone

   Use

   Entry vector for grna

   Tags

   Expression

   Mutation

   Promoter

   CmYLCV

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-scFv-p65-HSF1-Blast

   Plasmid

   #192652

   Purpose

   3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   scFv-p65-HSF1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE30-butelase1

   Plasmid

   #115430

   Purpose

   Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

   Depositor

   Joshua Mylne

   Article

   James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

   Insert

   butelase 1
   

   Use

   Tags

   6-His

   Expression

   Mutation

   Promoter

   T5

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

   Plasmid

   #86414

   Purpose

   nuclear split GFP11 reporter without mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-PUS1
   

   Use

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   NOP1pr

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. eiCBE

   Plasmid

   #227148

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R/H339A

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-OMP25

   Plasmid

   #141150

   Purpose

   Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpdCas9 VPR

   Plasmid

   #188509

   Purpose

   Expresses FLAG tagged SpdCas9 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2858

   Plasmid

   #143385

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ID3 (ID3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. miCBE

   Plasmid

   #205413

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with APOBEC3A and UGI driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

   Insert

   pU6__RNA*_pCAG_bpNLS_APOBEC3A(W104A)_XTEN_OgeuIscB*(D61A)_NLS_2xUGI_bGHployA_pCMV_mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D61A+E85R+H369R+S387R+S457R

   Promoter

   hU6, CAG, CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGem-PgpdA-Cre-TtrpC

    Plasmid

    #168784

    Purpose

    Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Insert

    Cre
    

    Use

    Cre/Lox and Synthetic Biology; Expression in fila…

    Tags

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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