2,223 results

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pDONR221-hmgn6

Plasmid

#192715

Purpose

Gateway entry vector encoding zebrafish hmgn6

Depositor

Summer Thyme

Insert

hmgn6 (hmgn6 Zebrafish)

Use

Gateway Cloning

Promoter

None

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1 Danio rerio CBS-pair domain of CNNM4

Plasmid

#170785

Purpose

Expresses pGEX-6P1 Danio rerio CBS-pair domain of CNNM4

Depositor

Kalle Gehring

Insert

CBS from CNNM4

Tags

GST

Expression

Bacterial

Mutation

NA

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Danio rerio PRL (Δprenylation motif)

Plasmid

#166417

Purpose

Express pET-15b(+) Danio rerio PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Danio rerio PRL (ptp4a3a Zebrafish)

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ptia1l-hsp-GFP

Plasmid

#177837

Purpose

Donor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9

Depositor

David Kingsley

Article

Wucherpfennig et al Nat Ecol Evol. 2022 Sep 1. pii: 10.1038/

Insert

tia1l-sgRNA target site-hsp70-EGFP

Use

CRISPR

Tags

EGFP

Promoter

hsp70

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-His-zfCsf1b

Plasmid

#168104

Purpose

Express soluble His-tagged zebrafish Csf1b in insect cells

Depositor

Petr Bartunek

Article

Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

Insert

Csf1b (csf1b Zebrafish)

Tags

6x His

Expression

Insect

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox5-PLAT-EGFP-6H

Plasmid

#187189

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000057273 (alox5a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubgenomic_D

Plasmid

#146143

Purpose

Mammalian Expression of tubgenomic

Depositor

Elisa Izaurralde

Article

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubgenomic

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34R-D87R-EGFP-6H

Plasmid

#187188

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

D34R, D87R

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32F-L33F-EGFP-6H

Plasmid

#187184

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

L32F, L33F

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-Y90A-V91A-EGFP-6H

Plasmid

#187185

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90A, V91A

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-L32A-L33A-EGFP-6H

Plasmid

#187183

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

L32A, L33A

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-Alox12-PLAT-EGFP-6H

Plasmid

#187190

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000069463 (alox12 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-D34K-D87K-EGFP-6H

Plasmid

#187187

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

D34K, D87K

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-npas4l

Plasmid

#164654

Purpose

for the synthesis of zebrafish npas4l mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

npas4l (npas4l Zebrafish)

Use

Mrna synthesis

Mutation

Please see depositor comments

Promoter

SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2z-etsrp

Plasmid

#164655

Purpose

for the synthesis of zebrafish etsrp mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

etsrp (etsrp Zebrafish)

Use

Mrna synthesis

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2z-lmo2

Plasmid

#164657

Purpose

for the synthesis of zebrafish lmo2 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

lmo2 (lmo2 Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-osr1-KI-donor

Plasmid

#184875

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Odd-Skipped Related Transcription Factor 1 5' homology arm
Odd-Skipped Related Transcription Factor 1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP

Use

CRISPR and Cre/Lox

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-col2a1a-KI-donor

Plasmid

#184876

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen2a1a 5' homology arm
Collagen2a1a 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP

Use

CRISPR and Cre/Lox

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-AA

Plasmid

#182157

Purpose

Knockin plasmid with homologous arm for making zMADM-AA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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KI-eab2-GG

Plasmid

#182158

Purpose

Knockin plasmid with homologous arm for making zMADM-GG zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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