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Plasmid#214673PurposeMAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice siteDepositorInsertMAPT (MAPT Human)
UseTagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9, Exon 10 with a mutation to intro…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL2 HNF4A P2 mut
Plasmid#60325PurposeMutated HNF4A P2 promoter region cloned into the pGL2-basic backbone. The -181 G to A mutation is in a HNF1alpha binding site and cosegregates with MODY.DepositorInsertHNF4A P2 mut (-181 G to A) (HNF4A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationThe -181 G to A mutation is in a HNF1alpha bindin…PromoterAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Plasmid#115684PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.DepositorInsertNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGL3-Control-E2F-3'UTR-Mut
Plasmid#21171DepositorInsertE2F 3'UTR Mutant (E2F1 Human)
UseLuciferaseTagsExpressionMammalianMutation2 miR binding sites mutated. Changed from GCACTTT…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
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phR-Luc
Plasmid#139644PurposeMammalian gene expressionDepositorInsertRenilla luciferase
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q, R261Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG223 PRKD1 3'UTR mut
Plasmid#12042DepositorInsertPRKD1 3'UTR mut (PRKD1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
XRE-pNL1.3[secNLuc]
Plasmid#182295PurposeSecreted Luciferase reporter for AhR activityDepositorInsertXenobiotic Response Element (CYP1A1 Human)
UseTagssecNlucExpressionMammalianMutationPromoterXREAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) NanoLuc
Plasmid#208576PurposeEmpty NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
Empty response element
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterPGK and miniPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Fluc/CMV/WPREdU3
Plasmid#136881PurposeReporter vector expression firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
10-4 cyclin E promoter
Plasmid#8458DepositorInserthuman cyclin E promoter (CCNE1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL2-356bp
Plasmid#16292DepositorInsertp53 promoter fragment (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationfragment -344 to +12 relative to first transcript…PromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-renilla
Plasmid#38235DepositorInsertEef2 5'UTR / Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationEef2 5' UTR fused to renilla ORFPromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG/Luci
Plasmid#139981PurposeExpresses luciferase under the promoter of CAG. Transgene flanked by AAV2 ITRDepositorInsertLuciferase
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ShhN-Ren
Plasmid#37677DepositorInsertshh (Shh Mouse)
UseTagsrenilla luciferaseExpressionMammalianMutationsoluble form (see comments)PromoterCMVAvailable sinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-p27Luc
Plasmid#23047DepositorInsertp27 (CDKN1B Human)
UseTagsLuciferaseExpressionMammalianMutationwt cDNAPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFbx15(-1172/+114)-Luc
Plasmid#13412DepositorInsertUpstream region of mouse Fbx15 gene
UseLuciferaseTagsExpressionMammalianMutationNonePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLuc-3'UTR
Plasmid#42094DepositorInsertZEB1 3UTR (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL2-200bp
Plasmid#16293DepositorInsertp53 promoter fragment (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationfragment -188 to +12 relative to first transcript…PromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL2-100bp
Plasmid#16294DepositorInsertp53 promoter fragment (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationfragment -88 to +12 relative to first transcripti…PromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pAG217 PDLIM5 3'UTR mut
Plasmid#12047DepositorInsertN18 3'UTR mut (PDLIM5 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-rev-erbα
Plasmid#186823PurposeFluorescent reporter for rev-erbα expressionDepositorInsertrev-erbα promoter
UseLuciferaseTagsExpressionMutationPromoterrev-erbα promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only