We narrowed to 4,130 results for: pCMV mammalian

1. MOBE3

   Plasmid

   #219943

   Purpose

   Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8e
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MOBE4

   Plasmid

   #219944

   Purpose

   Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. apt-ABE8.20

   Plasmid

   #219938

   Purpose

   Mammalian expression of apt-ABE8.20 protein (TadA8.20_32aa-linker_Com)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   TadA8.20_32-aa_Com
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

   Plasmid

   #50031

   Purpose

   Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   PM-SrtA(strep)
   

   Tags

   C-HA

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MOBE2

   Plasmid

   #219942

   Purpose

   Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-3'end_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TALE-free DddAtox 1397C

   Plasmid

   #221941

   Purpose

   Expresses mitochondrially targeted TALE-free DddAtox 1397C-UGI in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TALE-free DddAtox 1397N

   Plasmid

   #221942

   Purpose

   Expresses mitochondrially targeted TALE-free DddAtox 1397N-UGI in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-DddAtox 1397N-UGI-ATP5B 3'UTR
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TALE-free DddA6-11 1397C

   Plasmid

   #221943

   Purpose

   Expresses mitochondrially targeted TALE-free DddA6/11 1397C-UGI in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   DddA: T1413I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TALE-free DddA6 1397N

   Plasmid

   #221944

   Purpose

   Expresses mitochondrially targeted TALE-free DddA6 1397N-UGI in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-DddA6 1397N-UGI-ATP5B 3'UTR
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   DddA: Q1310R, S1330I, T1380I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TALE-free DddA11 1397N

    Plasmid

    #221945

    Purpose

    Expresses mitochondrially targeted TALE-free DddA11 1397N-UGI in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-DddA11 1397N-UGI-ATP5B 3'UTR
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1330I, A1341V, N1342S, E1370K, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. nNc-ABE

    Plasmid

    #194095

    Purpose

    CMV-TadA 8e-nNcCas9. A-to-G base editing in human cells.

    Depositor

    Zhanjun Li

    Article

    Liu et al Commun Biol. 2022 Nov 26;5(1):1296. doi:

    Insert

    TadA 8e-nNcCas9
    

    Expression

    Mammalian

    Mutation

    NcCas9 D16A

    Promoter

    CMV

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBiFC-CC155

    Plasmid

    #22015

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    CC155
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTALEN_v2 (NG)

    Plasmid

    #32190

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NG for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTALEN_v2 (HD)

    Plasmid

    #32192

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, HD for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMX232

    Plasmid

    #23018

    Depositor

    Linda Wordeman

    Article

    Wordeman et al J Cell Biol. 2007 Dec 3. 179(5):869-79.

    Insert

    RMLM
    

    Tags

    6H and mRFP1.0

    Expression

    Mammalian

    Mutation

    deleted MCAK amino acids 192-582

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTALEN_v2 (NI)

    Plasmid

    #32189

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NI for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTALETF_v2 (NN)

    Plasmid

    #32185

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NN for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTALEN_v2 (NN)

    Plasmid

    #32191

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NN for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTALETF_v2 (HD)

    Plasmid

    #32188

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, HD for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALETF_v2 (NG)

    Plasmid

    #32186

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NG for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTALETF_v2 (NI)

    Plasmid

    #32187

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NI for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVH321-1-Tier1-PhCMV-dCas9-3xNLS

    Plasmid

    #169595

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

    Plasmid

    #228721

    Purpose

    Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

    Plasmid

    #228727

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

    Plasmid

    #228728

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid

    #228733

    Purpose

    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

    Plasmid

    #228722

    Purpose

    Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid

    #228723

    Purpose

    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2 nCas9 (D16A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid

    #228724

    Purpose

    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

    Plasmid

    #228732

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

    Plasmid

    #228731

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTS2374-Tier1-PhCMV-Gal4_VP16

    Plasmid

    #169582

    Purpose

    Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    GAL4-transactivator
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGinB-8X(GGS)-dCas9-FLAG-NLS

    Plasmid

    #81205

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CMV+ episome

    Plasmid

    #169742

    Purpose

    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

    Plasmid

    #81206

    Purpose

    CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    pGinB-8X(GGS)-dCas9-FLAG-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDisplay-SNAPtag

    Plasmid

    #205094

    Purpose

    Display SNAPtag on the surface of mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    SNAPtag
    

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. nCas9-SpRY_P2A-mCherry

    Plasmid

    #219935

    Purpose

    Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-SpRY_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge