We narrowed to 11,082 results for: Kars;

1. pLVX-opto-hCaspase-8

   Plasmid

   #208786

   Purpose

   Inducibly expresses human Caspase 8 fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Caspase 8 (CASP8 Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   CARD domain is removed

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTP-PCR C3

   Plasmid

   #189620

   Purpose

   Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

   Insert

   Reduced Mitochondrial Genome of Thalassiosira pseudonana
   

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Mutation

   Removed repeat region, 10-kbp deletion, and minor…

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-RFP-P2A-MCS (Neo)

   Plasmid

   #89182

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-FLInChR-mVenus

   Plasmid

   #119298

   Purpose

   FLinChR is the fusion signal sequence and transmembrane (TM) domain of Neurexin 1B-delta to ChR2E123T/T159C for inversion ChR2 to an optogenetic inhibitor

   Depositor

   Alla Karpova

   Article

   Brown et al Cell. 2018 Oct 16. pii: S0092-8674(18)31

   Insert

   Nx1BTM-FCS-ChR2ET/TC-mVenus
   

   Use

   AAV

   Tags

   mVenus

   Promoter

   CAG

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK1

   Plasmid

   #111722

   Purpose

   to express human BLM protein in yeast cells

   Depositor

   Ian Hickson

   Article

   Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

   Insert

   BLM (BLM Human)
   

   Tags

   hexahistidine

   Expression

   Yeast

   Mutation

   a C-terminal hexahistidine tag was added, a yeast…

   Promoter

   GAL1

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. BSD-CryC

   Plasmid

   #209672

   Purpose

   Expresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cells

   Depositor

   Sanjeevi Sivasankar

   Article

   Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Lentiviral

   Tags

   Split-TurboID-C and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC5

   Plasmid

   #188602

   Purpose

   Superior Conjugative Plasmid to Diverse Yeast

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC5GGv1

   Plasmid

   #194645

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXJ40 GADD34 (Delta Pest1)

    Plasmid

    #78096

    Purpose

    mammalian expression of GADD34 Delta Pest1

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    deletion of PEST1

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXJ40 GADD34 (514-674aa)

    Plasmid

    #75480

    Purpose

    mammalian expression of GADD34 514-674 aa

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    expresses aa 514-674

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pXJ40 GADD34 (1-513aa)

    Plasmid

    #75479

    Purpose

    mammalian expression of GADD34 1-513 aa

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    expresses aa 1-513

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-OSF-BECN1

    Plasmid

    #99328

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    Beclin 1 (BECN1 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-OSF-VPS34

    Plasmid

    #99327

    Purpose

    Component for mammalian expression of recombinant PI3KC3-C1 complex

    Depositor

    James Hurley

    Article

    Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

    Insert

    VPS34 (PIK3C3 Human)
    

    Tags

    Strep-Strep-Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

15. PXL

    Plasmid

    #75349

    Purpose

    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK TT 3xFLAG SMAD4 neo

    Plasmid

    #83273

    Purpose

    Lentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    SMAD4 (SMAD4 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pDEINO1

    Plasmid

    #179472

    Purpose

    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Inserts

    ori
    cat
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Circular 100,50 (RAB7A)

    Plasmid

    #170117

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 100,50 guide RNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTSK561

    Plasmid

    #190816

    Purpose

    For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

    Depositor

    Tatiana Karpova, Gunjan Mehta

    Article

    Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

    Insert

    HaloTag
    

    Use

    Bacterial cloning vector

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcdna3 Flag JIP3b

    Plasmid

    #53458

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only