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Human INPP5A

Plasmid

#129587

Purpose

Expression of human INPP5A in bacteria

Depositor

Christophe Erneux

Article

Erneux et al Eur J Biochem. 1995 Dec 1;234(2):598-602

Insert

Type I inositol 5-phosphatase (INPP5A Human)

Tags

Maltose binding protein

Expression

Bacterial

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

Plasmid

#113695

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn1-3 (CG11739)

Plasmid

#110642

Purpose

Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG11739 (CG11739 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1-d207

Plasmid

#114050

Purpose

Expression of exogenous LacI-GFP-HEC1 delta 207

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1-d207 (NDC80 Human)

Expression

Mammalian

Mutation

HEC1 delta 207

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-hygro-eGFP-HEC1-d207

Plasmid

#114051

Purpose

Expression of exogenous eGFP HEC1 delta 207

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

eGFP-HEC1-d207 (NDC80 Human)

Expression

Mammalian

Mutation

HEC1 delta 207

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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hASXL(1-115)-luciferase

Plasmid

#106427

Purpose

Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

addition sex combs like 1 ASXL1 (ASXL1 Human)

Tags

luciferase

Expression

Mammalian

Mutation

amino acids 1-115 fused to luciferase

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS K283D Q287D

Plasmid

#79068

Purpose

Express owl monkey TRIMCyp K283D, Q287D in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey) (K283D, Q287D)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

Plasmid

#79030

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

deleted amino acid 294-515 from frican green monk…

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFUPI hT5Cyp-H126Q

Plasmid

#80281

Purpose

Expresses human TRIM5 CypA fusion with H126Q mutation in CypA

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion (TRIM5, PPIA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

H126Q mutation in CypA

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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