165,652 results

1. tetO-PBX1

   Plasmid

   #170699

   Purpose

   doxycycline-inducible overexpression of PBX1

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   PBX1 (PBX1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK290 (mAID-mClover-Hygro)

   Plasmid

   #72828

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCE048-SiT-Cas12a

   Plasmid

   #128124

   Purpose

   Express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-Lime

   Plasmid

   #191255

   Purpose

   Bacterial expression plasmids for pH sensitive fluorescent protein Lime

   Depositor

   Robert Campbell

   Article

   Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

   Insert

   Lime
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK18sB

   Plasmid

   #177838

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. U6-5'+3' MS2-CircRNA

   Plasmid

   #213752

   Purpose

   For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Tubulin-C-18

   Plasmid

   #55148

   Purpose

   Localization: Microtubules, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-ERT2-iCre-ERT2

   Plasmid

   #178059

   Purpose

   Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ERT2-iCre-ERT2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human synapsin 1 (Syn) promoter

   Available Since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_478

   Plasmid

   #179096

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (SpG)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p5xATF6-GL3

    Plasmid

    #11976

    Purpose

    Reporter gene with five ATF6 sites that is highly sensitive to ER stress

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    5xATF6 binding site
    

    Tags

    Luc and c-fos promoter

    Expression

    Mammalian

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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