162,408 results

1. pAL119-TK

   Plasmid

   #21911

   Depositor

   Maria Castro

   Article

   Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

   Insert

   HSV Type 1 thymidine kinase
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 3XFlag DVL2 (WT)

   Plasmid

   #24802

   Depositor

   Jeff Wrana

   Article

   Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

   Insert

   DVL2 (DVL2 Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217515

   Purpose

   Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ELP-TEMP/pcDNA3.1(-)

   Plasmid

   #178434

   Purpose

   Genetically encoded ratiometric fluorescent thermometer

   Depositor

   Takeharu Nagai

   Article

   Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

   Insert

   ELP-TEMP
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. f-CTS-EGFP

   Plasmid

   #86064

   Purpose

   A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains ciliary targeting signal (3870-3887 amin…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SlugCas9

   Plasmid

   #163793

   Purpose

   Expresses SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHAGE-EZH2

   Plasmid

   #116738

   Purpose

   Lentiviral expression of EZH2

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV-CMV-DsRed-IRES-d1EGFP

   Plasmid

   #41942

   Purpose

   Retroviral expression of DsRed in mammalian cells

   Depositor

   Stephen Elledge

   Article

   Yen et al Science. 2008 Nov 7;322(5903):918-23. do

   Insert

   DsRed
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   May 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge