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We narrowed to 115,120 results for: CIL

Showing: 1461 - 1480 of 115120 results

  1. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

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  2. pCas9FnCpf1GG

    Plasmid
    #131013
    Purpose
    Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    GFP expression cassette
    direct repeat of FnCas12a
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131013-plasmid-map-sequence-id-257083

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  3. pVR2

    Plasmid
    #84719
    Purpose
    E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    12 nt stretch lacking A's inserted before 5&…
    Promoter
    Available since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84719-plasmid-map-sequence-id-167481

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  13. pG2J

    Plasmid
    #102882
    Purpose
    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Inserts
    OsTIR1
    mGFP-AID
    Use
    Tags
    AID(71-114)
    Expression
    Yeast
    Mutation
    Promoter
    ADH1 and TEF1
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102882-plasmid-map-sequence-id-199289

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  14. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

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  16. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid
    #80768
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
    Depositor
    Kazuhisa Nakayama
    Article
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    80768-plasmid-map-sequence-id-171038

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  17. pLentiRNAGuide_003

    Plasmid
    #192505
    Purpose
    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:
    Insert
    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192505-plasmid-map-sequence-id-375155

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  19. CoV2-M-IRES-E

    Plasmid
    #177938
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177938-plasmid-map-sequence-id-429078

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Showing: 1461 - 1480 of 115120 results