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Addgene

We narrowed to 8,865 results for: BLI;

Showing: 3651 - 3700 of 8865 results
  1. pHL 1915

    Plasmid
    #53022
    Purpose
    AmpR + PLlacO1fims::gfp::Asp terminator + CamR + ColE1 (PLlacO1fimS is a fused PLlacO-1 and fimS promoter)
    Depositor
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and fimS fusion
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. m2ap

    Plasmid
    #50051
    Purpose
    expresses toxoplasma gondii MIC2 associated protein (m2ap) in mammalian cells
    Depositor
    Insert
    M2AP
    Use
    Tags
    HIS
    Expression
    Mammalian
    Mutation
    starts at aa47; N127R, S158A, S177N, N357S, N463S…
    Promoter
    CMV
    Available since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. 3EOZ

    Plasmid
    #51349
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PP-PGAM2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pRKVSV-PTEN C124A

    Plasmid
    #50539
    Depositor
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    2X VSV
    Expression
    Mammalian
    Mutation
    C124A
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pRKVSV-PTEN G129E

    Plasmid
    #50542
    Depositor
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    2X VSV
    Expression
    Mammalian
    Mutation
    G129E
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. TATDN3

    Plasmid
    #39086
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    TATDN3 (TATDN3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  7. NANP

    Plasmid
    #38993
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    NANP (NANP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  8. ZADH2

    Plasmid
    #39006
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    ZADH2 (ZADH2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  9. MYNN

    Plasmid
    #38909
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MYNN (MYNN Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  10. RHPN2

    Plasmid
    #38919
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    RHPN2 (RHPN2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  11. MPDZ

    Plasmid
    #38952
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MPDZ (MPDZ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  12. MPDZ

    Plasmid
    #38953
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MPDZ (MPDZ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  13. MPDZ

    Plasmid
    #38955
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MPDZ (MPDZ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  14. MPDZ

    Plasmid
    #38956
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MPDZ (MPDZ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  15. MPDZ

    Plasmid
    #38957
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    MPDZ (MPDZ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  16. ZADH2

    Plasmid
    #38848
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    ZADH2 (Zadh2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  17. OXSM

    Plasmid
    #38860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    OXSM (Oxsm Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  18. RTN4IP1

    Plasmid
    #38905
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    RTN4IP1 (RTN4IP1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  19. PP-MetAP

    Plasmid
    #40745
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PP-MetAP
    Use
    Tags
    6xHis TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 14, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  20. pLenti4/V5-DEST-P85(E439del)

    Plasmid
    #40221
    Depositor
    Insert
    P85(E439del) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deleted amino acid 439
    Promoter
    CMV
    Available since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
  21. Rac1 in pAcG2T

    Plasmid
    #38164
    Depositor
    Insert
    Rac1 (RAC1 Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    polyhedron
    Available since
    Aug. 16, 2012
    Availability
    Academic Institutions and Nonprofits only
  22. Bcr in pAcG2T

    Plasmid
    #38163
    Depositor
    Insert
    BCR (BCR Human)
    Use
    Tags
    GST
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only
  23. pDC10

    Plasmid
    #75077
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (H6EcPurD)
    Insert
    phosphoribosylglycinamide synthetase (purD Escherichia coli)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    adds unique SacII site at Thr95-Ala96 by a silent…
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
  24. pSBBi-RP CCS1

    Plasmid
    #139162
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes.
    Depositor
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
  25. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
  26. pSB-Bleo Gal4 CCS1

    Plasmid
    #139189
    Purpose
    SB-transposon with constitutive bleomycin resistance and Gal4-inducible coffee caffeine synthase 1 (CCS1).
    Depositor
    Insert
    caffeine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Gal4-inducible CMV and STAT3
    Available since
    Availability
    Academic Institutions and Nonprofits only
  27. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

    Plasmid
    #182888
    Purpose
    Mammalian expression of PVALB mouse mAb HC gene
    Depositor
    Insert
    anti-PVALB (human) heavy chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  28. Pet28a_Human_H3.1

    Plasmid
    #42631
    Depositor
    Insert
    Human H3.1 (H3C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
  29. Halo-TOMM20-N-10

    Plasmid
    #123284
    Purpose
    Localization: Outer Mitochondrial Membrane
    Depositor
    Insert
    TOMM20 (TOMM20 Human)
    Use
    Tags
    HaloTag7
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  30. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Insert
    Ubiquitin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  31. pC1-CMV-mito-DAAO

    Plasmid
    #141132
    Purpose
    Expresses DAAO with mitochondrial localization signal in mammalian cells
    Depositor
    Insert
    mito-DAAO
    Use
    Tags
    2 mitochondrial localization signals and 7xHis
    Expression
    Mammalian
    Mutation
    deleted DAAO amino acids 367-368
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  32. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  33. pLV-DBNF-mCherry

    Plasmid
    #187195
    Purpose
    To express the mouse BDNF tagged with the FP mCherry
    Depositor
    Insert
    BDNF
    Use
    Lentiviral
    Tags
    fluorescent protein mCherry
    Expression
    Mammalian
    Mutation
    The codons have been optimized for better express…
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  34. pCW57.1 CMYC

    Plasmid
    #164145
    Purpose
    Doxycycline inducible expression of c-MYC
    Depositor
    Insert
    MYC (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Doxycycline inducible promoter
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  35. pcDNA3.1-Flag

    Plasmid
    #208051
    Purpose
    Empty vector for mammalian expression, insert a Flag tag (with ATG) at XhoI site of pcDNA3.1 (-), xhoI site at 5' of flag tag destroyed, xhoI site at 3' of flag tag retained. XhoI in-frame with Flag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  36. Pet28a_Human_H4

    Plasmid
    #42633
    Depositor
    Insert
    Human H4 (H4C1 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
  37. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid
    #50478
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Has Service
    AAV5
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
  38. pAAV-hSyn-DIO-EGFP

    Plasmid
    #50457
    Purpose
    Double floxed EGFP under the control of human synapsin promoter
    Depositor
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV8
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    human Synapsin 1
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
  39. pFLAG-6a-Ataxin3Q22

    Plasmid
    #22126
    Depositor
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2009
    Availability
    Academic Institutions and Nonprofits only
  40. pAAV-FLEX-tdTomato

    Plasmid
    #28306
    Depositor
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
  41. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  42. FLAG-hMYOD1 (#794)

    Plasmid
    #78329
    Purpose
    Overexpression of human MYOD1 ORF with a N-terminal FLAG tag
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
  43. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Has Service
    AAV PHP.eB and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Expression
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  44. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Insert
    Peptide: N-glycosidase F
    Use
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  45. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Insert
    ER-SPLICSs-cFAST10
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  46. pLVX ALIX-GFP WT

    Plasmid
    #138582
    Purpose
    Expresses fluorescent ALIX in mammalian cells
    Depositor
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  47. pQC V5 MCS IRES G418

    Plasmid
    #110345
    Purpose
    γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  48. pCMV14_Fibrillarin

    Plasmid
    #73061
    Purpose
    expression clone for human Fibrillarin with C-terminal 3xFLAG-tag
    Depositor
    Insert
    Fibrillarin (FBL Human)
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  49. pML104-KanMx4

    Plasmid
    #83476
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.
    Depositor
    Insert
    KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
  50. ORFtag_TetR-SD_Frame1

    Plasmid
    #220981
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1
    Depositor
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK promoter
    Available since
    June 28, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 3651 - 3700 of 8865 results