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Showing: 3651 - 3700 of 4075 results

  3. ZJOM27

    Plasmid
    #133641
    Purpose
    Integration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pCUP1-GFP-Pho8 (PHO8 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133641-plasmid-map-sequence-id-268964

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  4. ZJOM144

    Plasmid
    #133630
    Purpose
    Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-SS-GFP-HDEL
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133630-plasmid-map-sequence-id-269548

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  5. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Article
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  11. pPMVAu5 (R)

    Plasmid
    #98296
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98296-plasmid-map-sequence-id-201300

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  13. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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  24. pCSN074

    Plasmid
    #166731
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166731-plasmid-map-sequence-id-332203

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  27. pHB4

    Plasmid
    #53462
    Purpose
    This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.
    Depositor
    Elizabeth Conibear
    Article
    Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11
    Insert
    yeGFP-Snc1-Suc2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    53462-plasmid-map-sequence-id-83347

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  30. pCSN069

    Plasmid
    #166728
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1
    Use
    CRISPR
    Tags
    Mxi1
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166728-plasmid-map-sequence-id-332209

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  33. pDA125

    Plasmid
    #74249
    Purpose
    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).
    Depositor
    Serge Pelet
    Article
    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10
    Insert
    Inducible peptide of the dPSTR
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    pSTL1
    Available since
    May 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74249-plasmid-map-sequence-id-140105

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  36. pSP571

    Plasmid
    #139498
    Purpose
    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pGPD Cas9 / sgRNA (STL162)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pGPD
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139498-plasmid-map-sequence-id-275585

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  37. GFP-X3

    Plasmid
    #115568
    Purpose
    Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Three repeats of the Gcn5 consensus motif are tag…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115568-plasmid-map-sequence-id-226342

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  40. pJT3

    Plasmid
    #98304
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    TRPL3AcNES1>TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98304-plasmid-map-sequence-id-190175

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Showing: 3651 - 3700 of 4075 results