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pUPD3_FULLPRM_PT7

Plasmid

#243917

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7lac

Plasmid

#243918

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo1_Stuffer1_Stuffer2

Plasmid

#243896

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1 and Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo2_Stuffer2_Stuffer1

Plasmid

#243897

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2 and Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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ptetgRNA-FRT

Plasmid

#243872

Purpose

sgRNA expression vector under tetR transcriptional control.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

sgRNA_FRT

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM139

Plasmid

#247638

Purpose

to generate mRNA for membrane mGold2t for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mGold2t

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_1FL0

Plasmid

#234216

Purpose

Heterologous protein expression of aminoacyl tRNA synthase complex-interacting multifunctional protein 1 in Escherichia coli

Depositor

Christopher Bahl

Insert

1FL0 (AIMP1 Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_LacI_pLac-sgRNA-1

Plasmid

#231408

Purpose

sgRNA-1 expression from an IPTG-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
LacI

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_AraC_pBAD-sgRNA-1

Plasmid

#231407

Purpose

sgRNA-1 expression from an arabinose-inducible promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
AraC

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB1465

Plasmid

#218625

Purpose

Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Adr1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1401

Plasmid

#218615

Purpose

Express Pex13, Pex14, Pex17, Pex2, Pex10, and Pex12

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex13

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1469

Plasmid

#218588

Purpose

Express peroxisomal MvaSp and MvaEp to convert AcCoA to mevalonate

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

MvaS

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_MYC9

Plasmid

#223897

Purpose

4G-cloning donor for C-terminal MYC9 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

Myc9

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_PK6

Plasmid

#223898

Purpose

4G-cloning donor for C-terminal PK6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

PK6

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG

Plasmid

#223895

Purpose

4G-cloning donor for C-terminal FLAG tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_HALO

Plasmid

#223899

Purpose

4G-cloning donor for C-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6

Plasmid

#223871

Purpose

4G-cloning donor for N-terminal His6 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6

Use

Golden-gate donor

Tags

6xHis

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-3C

Plasmid

#223873

Purpose

4G-cloning donor for N-terminal His6-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_HALO

Plasmid

#223885

Purpose

4G-cloning donor for N-terminal HALO tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

HALO

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pD(Nt)_GFP

Plasmid

#223880

Purpose

4G-cloning donor for N-terminal GFP tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pD(Nt)_TS-3C

Plasmid

#223883

Purpose

4G-cloning donor for N-terminal TwinStrep-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

TwinStrep-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_GFP-3C

Plasmid

#223882

Purpose

4G-cloning donor for N-terminal GFP-3C tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

GFP-3C

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_His8

Plasmid

#223886

Purpose

4G-cloning donor for C-terminal His8 tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pD(Ct)_TS

Plasmid

#223891

Purpose

4G-cloning donor for C-terminal TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-TS

Plasmid

#223892

Purpose

4G-cloning donor for C-terminal 3C-TwinStrep tag

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

3C-TwinStrep

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Bneu-10

Plasmid

#205215

Purpose

Unit Bactoneuron-10, expresses E2-crimson

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Inserts

E2-Crimson
LuxR

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pZLrhaB2plus

Plasmid

#211787

Purpose

pSCrhaB2plus with modifications of resistance cassette to CmR

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

CmR

Expression

Bacterial

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE-RA1.0

Plasmid

#208297

Purpose

Expresses ABE-RA1.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA1.0-SpCas9 D10A

Expression

Mammalian

Mutation

D108G

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA1.1

Plasmid

#208298

Purpose

Expresses ABE-RA1.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA1.1-SpCas9 D10A

Expression

Mammalian

Mutation

D108G, K161N

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJS237

Plasmid

#200713

Purpose

ADH2 terminator from yeast (MoClo 3´U +Ter)

Depositor

Jens Boch

Article

Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

Insert

ADH2 Terminator

Use

Synthetic Biology

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-ISceI-DSCP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194517

Purpose

DSCP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpM-CtDCP1_X

Plasmid

#148006

Purpose

Bacterial Expression of CtDCP1

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP1

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpGB1-CtNot2_X

Plasmid

#147969

Purpose

Bacterial Expression of CtNot2

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtNot2

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpM-CtPop2_X

Plasmid

#147970

Purpose

Bacterial Expression of CtPop2

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtPop2

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NpS-CtNot3_X

Plasmid

#147971

Purpose

Bacterial Expression of CtNot3

Depositor

Elisa Izaurralde

Article

Raisch et al J Struct Biol. 2018 Dec;204(3):388-395.

Insert

CtNot3

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2_M

Plasmid

#146937

Purpose

Insect Expression of DmPAN2

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN2 (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3_M

Plasmid

#146938

Purpose

Insect Expression of DmPAN3

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

DmPAN3 (CG11486 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB11

Plasmid

#185422

Purpose

Full-length KAP60/SRP1

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

Insert

KAP60

Expression

Yeast

Promoter

endogenous

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK29-wzzpHS2

Plasmid

#172971

Purpose

pWSK29 with S. flexneri wzzpHS2 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzPHS2 gene from Shigella flexneri

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWSK29-wzzBSF

Plasmid

#172967

Purpose

pWSK29 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Shigella flexneri

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-luc-miR-1

Plasmid

#171556

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-1

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-1

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_EF_t45582

Plasmid

#154037

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator 45582

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AC_pNub

Plasmid

#154038

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Nub

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_EF_tSVP

Plasmid

#154041

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator SVP

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AC_pPbt

Plasmid

#154042

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Pbt

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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