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Showing: 1461 - 1480 of 11043 results

  1. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  5. pPHR-mCherry-FUSN-mHP1alpha

    Plasmid
    #181892
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181892-plasmid-map-sequence-id-359691

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  7. pPHR-mCherry-nE-mHP1alpha

    Plasmid
    #181890
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181890-plasmid-map-sequence-id-359697

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  8. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Kristijan Ramadan, Ivana Marinovic-Terzic
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  9. pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

    Plasmid
    #181893
    Purpose
    Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181893-plasmid-map-sequence-id-359709

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  10. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid
    #181891
    Purpose
    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
    Depositor
    Karsten Rippe
    Article
    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.
    Insert
    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mouse, Mustard Weed)
    Use
    Tags
    PHR-mCherry
    Expression
    Mammalian
    Mutation
    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion
    Promoter
    CMV
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    181891-plasmid-map-sequence-id-359705

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  15. p5059

    Plasmid
    #198154
    Purpose
    bacterial expression of steC::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsteC steC::L16::HaloTag::HA
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198154-plasmid-map-sequence-id-386983

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  16. pAC32 [TU#839]

    Plasmid
    #16079
    Depositor
    Martin Chalfie
    Insert
    mec-2 intron 9
    Use
    Tags
    YFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 23, 2008
    Availability
    Academic Institutions and Nonprofits only
    16079-plasmid-map-sequence-id-8472

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  17. pME-QF2

    Plasmid
    #83307
    Purpose
    codes for QF2 (truncated QF) in a gateway middle entry backbone.
    Depositor
    Marnie Halpern
    Insert
    QF
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    83307-plasmid-map-sequence-id-173284

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  18. p4305

    Plasmid
    #198150
    Purpose
    bacterial expression of sifA::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198150-plasmid-map-sequence-id-388083

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  19. p4286

    Plasmid
    #198152
    Purpose
    bacterial expression of sseJ::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198152-plasmid-map-sequence-id-388084

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  20. p4118

    Plasmid
    #198151
    Purpose
    bacterial expression of sseF::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198151-plasmid-map-sequence-id-388048

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Showing: 1461 - 1480 of 11043 results