We narrowed to 5,102 results for: codon optimized

1. pCG_SARS-CoV2 NSP15 C-V5-tag

   Plasmid

   #179975

   Purpose

   Mammalian expression vector for SARS-CoV-2 Nsp15, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-2 Nsp15 (ORF1ab Synthetic)
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

   Plasmid

   #114093

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548V and N552R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJ206

   Plasmid

   #162679

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E226T; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG_SARS-CoV1 NSP15 C-V5-tag

   Plasmid

   #179964

   Purpose

   Mammalian expression vector for SARS-CoV-1 Nsp15, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-1 Nsp15 (ORF1ab Synthetic)
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFGC-I2Cas9

   Plasmid

   #173158

   Purpose

   Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Inserts

   hSpCas9
   pNAP:dsRED:tNOS
   pMAS:BAR:tMAS
   ICU2 upstream regulatory region
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Plant

   Mutation

   Contains a D169N mutation with no visible effect …

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG_batCoV RaTG13 NSP1 C-V5-tag

   Plasmid

   #179982

   Purpose

   Mammalian expression vector for RaTG13 Nsp1, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   RaTG13 Nsp1
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCG_SARS-CoV2 NSP1 C-V5-tag

   Plasmid

   #179972

   Purpose

   Mammalian expression vector for SARS-CoV-2 Nsp1, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-2 Nsp1 (ORF1ab Synthetic)
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCJ211

   Plasmid

   #162684

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   deltaK2:G19; codon optimized for expression in E.…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCG_SARS-CoV1 M C-V5-tag

   Plasmid

   #179967

   Purpose

   Mammalian expression vector for SARS-CoV-1 M, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-1 M (M Synthetic)
   

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCG_SARS-CoV1 NSP1 C-V5-tag

    Plasmid

    #179961

    Purpose

    Mammalian expression vector for SARS-CoV-1 Nsp1, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 Nsp1 (ORF1ab Synthetic)
    

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCG_batCoV RaTG13 NSP15 C-V5-tag

    Plasmid

    #179984

    Purpose

    Mammalian expression vector for RaTG13 Nsp15, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    RaTG13 Nsp15
    

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid

    #114080

    Purpose

    CAG promoter expression plasmid for human codon optimized AsBE1.4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid

    #114077

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R/S542R/K607R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCG_batCoV RaTG13 M C-V5-tag

    Plasmid

    #179986

    Purpose

    Mammalian expression vector for RaTG13 M, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    RaTG13 M
    

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCJ197

    Plasmid

    #162670

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S131G; codon optimized for expression in E. coli …

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

    Plasmid

    #114083

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.3

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid

    #114071

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid

    #114079

    Purpose

    CAG promoter expression plasmid for human codon optimized AsBE1.1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCG_SARS-CoV1 NSP7 C-V5-tag

    Plasmid

    #179963

    Purpose

    Mammalian expression vector for SARS-CoV-1 Nsp7, V5-tagged. Sequence codon-optimized.

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-1 Nsp7 (ORF1ab Synthetic)
    

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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