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pG12-Rluc8

Plasmid

#170272

Purpose

Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

RLuc8

Use

Tags

Expression

Mammalian

Mutation

Promoter

12xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Sox2

Plasmid

#101761

Purpose

Sox2 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

SRY-box 2 promoter (SOX2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-TGFb1

Plasmid

#101762

Purpose

TGFb1 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

transforming growth factor beta 1 promoter (TGFB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-p57

Plasmid

#101760

Purpose

p57 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

cyclin dependent kinase inhibitor 1C promoter (CDKN1C Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Proximal Promoter-FLuc2P

Plasmid

#154259

Purpose

Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Proximal Promoter (PPARGC1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human PGC-1alpha Proximal Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Distal Promoter-FLuc2P

Plasmid

#154260

Purpose

Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Distal Promoter (PPARGC1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human PGC-1alpha Distal Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pIGF1_P2 Promoter-FLuc

Plasmid

#154267

Purpose

Firefly luciferase reporter containing the downstream Human IGF-1 P2 Promoter.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

IGF-1 P2 Promoter (IGF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human IGF-1 P2 Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pC0090

Plasmid

#181954

Purpose

Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xTOPFlash

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pC0091

Plasmid

#181955

Purpose

Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xFOP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV/R/U5

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV intron renilla TRICK CTE polyA

Plasmid

#84443

Purpose

TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

Renill-TRICK

Use

Luciferase; Frt

Tags

Expression

Mammalian

Mutation

Promoter

Tet-CMV

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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C239-E03: attB2r-IRES-ffLuc2-pA-attB3

Plasmid

#162919

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase with upstream IRES sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Ydf NanoLuc

Plasmid

#225656

Purpose

Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

Depositor

Neha Kamat

Article

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NanoLuciferase

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Mutation

Promoter

PydfJ115

Available since

Dec. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE1 (minus strand)

Plasmid

#91842

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0619)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE1 (minus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (plus strand)

Plasmid

#91848

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0625)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (plus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE2 (minus strand)

Plasmid

#91843

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0620)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE2 (minus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Perfect match to reference sequence

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sNluc2_STOP

Plasmid

#186767

Purpose

An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Use

Tags

Expression

Mutation

Premature stop codon at residue 16

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIGF1_P1 Promoter-FLuc

Plasmid

#154266

Purpose

Firefly luciferase reporter containing the canonical Human IGF-1 P1 Promoter.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

IGF-1 canonical P1 Promoter (IGF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human IGF-1 P1 Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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