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LHP306 - Ub - no Lys

Plasmid

#32150

Depositor

Linda Hicke

Article

Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

Promoter

CUP1

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pKPY514

Plasmid

#62598

Purpose

Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr251Gly-EcPheRS

Use

Tags

6xHis

Expression

Bacterial

Mutation

Thr251Gly

Promoter

T5

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Use

Tags

Expression

Insect

Mutation

Promoter

hsp70

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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LT1_34_sfpHluorin

Plasmid

#177707

Purpose

Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

pTEF1-sfpHluorin

Use

Used as donor in genome integration after lineari…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1-mMcl-1

Plasmid

#25717

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (Mcl1 Mouse)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
YN2_1_IL50_HOlocus

Plasmid

#194426

Purpose

Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT84_RPL13A

Plasmid

#177712

Purpose

Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Use

Tags

Knob

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a.TM1363

Plasmid

#11420

Depositor

Sung-Hou Kim

Article

Shin et al J Mol Biol. 2004 Jul 30. 341(1):227-39.

Insert

peptide release factor I (prfA Thermotoga maritima)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pOSV00157

Plasmid

#222937

Purpose

Kill switch plasmid for DNA sensor construction using Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

TxpA-RatA toxin-antitoxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Phyperspank

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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LT2_15_UPRpro_X2site

Plasmid

#194427

Purpose

Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

UPRpro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_33_PyruPro_X2site

Plasmid

#194429

Purpose

Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruPro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_34_EthPro_X2site

Plasmid

#194430

Purpose

Plasmid for genome integration in X2 site expressing ResPro (Ethanol consumption Probe)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

EthPro

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Apex2-Venus-CAAX

Plasmid

#168511

Purpose

Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

Depositor

Franck Polleux

Article

Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

Insert

Apex2

Use

Tags

Venus-CAAX

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip

Plasmid

#178050

Purpose

GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-TRAIL

Plasmid

#10953

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

Tumor necrosis factor alpha related apoptosis inducing ligand (TNFSF10 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

NA

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT3

Plasmid

#60040

Purpose

Mammalian expression of FLAG-tagged mouse TUT3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT3 (Papd5 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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