We narrowed to 10,494 results for: nar

1. pLVTHM-mSox17-3xflag

   Plasmid

   #206377

   Purpose

   Expresses 3xflag fused mouse SOX17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox17 (Sox17 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. JN302: pMVP/SB/Puro-DEST

   Plasmid

   #121865

   Purpose

   pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Sleeping beauty transposon, pmvp gateway destinat…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA 1 GAPDH

   Plasmid

   #83808

   Purpose

   Express Sg-1 sgRNA targeting GAPDH

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   SgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LB701: pMVP/PB/mCherry-DEST

   Plasmid

   #121871

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. II701: pMVP (L1-L4) cTnT promoter

   Plasmid

   #121690

   Purpose

   pMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   cTnT promoter (Cardiomyocyte specific)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LC301: pMVP/PB/Zeo-DEST

   Plasmid

   #121877

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LC101: pMVP/PB/Neo-DEST

   Plasmid

   #121875

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW17 - pAdapter

   Plasmid

   #174664

   Purpose

   Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Insert

   MS2 coat protein
   

   Use

   Tags

   λCI

   Expression

   Bacterial

   Mutation

   V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

   Promoter

   Synthetic Constitutive Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. IY601: pMVP/Lenti/Blast-DEST

   Plasmid

   #121849

   Purpose

   pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Lentiviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer-rat EF1 alpha

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CMV-NLS-dCas9-NLS

    Plasmid

    #99908

    Purpose

    For the expression of NLS-dCas9-NLS

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Insert

    NLS-dCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2a-copGFP(+HA)

    Plasmid

    #83806

    Purpose

    Express copGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    copGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-hCas9-T2A-mStrawberry

    Plasmid

    #187224

    Purpose

    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Inserted T2A-mStrawberry at 3' terminal of M…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET22b_EcSSB_2

    Plasmid

    #78204

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-

    Insert

    ssb (W88C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    W88C (please see depositor comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. LC001: pMVP/PB/Hygro-DEST

    Plasmid

    #121876

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLin28A-myc-T2A-tagRFP

    Plasmid

    #140267

    Purpose

    Expresses LIN28A in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

    Insert

    LIN28A (LIN28A Human)
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. IF101: pMVP (L1-L4) EF1a promoter

    Plasmid

    #121687

    Purpose

    pMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    EF1a promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdUA-lacIN

    Plasmid

    #141079

    Purpose

    Plasmid vector for testing DNA binding proteins by the I-Block assay.

    Depositor

    Antal Kiss

    Article

    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

    Type

    Empty backbone

    Use

    Vector for the i-block assay designed to study dn…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Escherichia coli lacIN promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. isoform PKC lambda (JCE590)

    Plasmid

    #89775

    Purpose

    yeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Article

    Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

    Insert

    PKC lambda (Prkci Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCH6 - pBait-ChiX

    Plasmid

    #174662

    Purpose

    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Insert

    ChiX
    

    Use

    Tags

    1xMS2 hairpin

    Expression

    Bacterial

    Mutation

    Promoter

    pBad

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. HB401: pMVP (L1-L4) CMV promoter

    Plasmid

    #121686

    Purpose

    pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    CMV promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28b-His-hCE

    Plasmid

    #112710

    Purpose

    Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNGTT (RNGTT Human)
    

    Use

    Tags

    6xHis and Stop codons placed after RNGTT sequence…

    Expression

    Bacterial

    Mutation

    R594H

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pOPO685

    Plasmid

    #195300

    Purpose

    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and native array with PaqCI entry site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUD628

    Plasmid

    #103018

    Purpose

    expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crADE2-3.S (ADE2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CMV-hCas9-T2A-miRFP670

    Plasmid

    #187223

    Purpose

    Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pSB700 HL1gR4

    Plasmid

    #124450

    Purpose

    Expresses gRNA HL1 gR4 in the pSB700 backbone

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    HL1 gR4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge