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Plasmid#179212PurposeGolden Gate entry vector; 3rd gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only
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pYPQ134-tRNA
Plasmid#179213PurposeGolden Gate entry vector; 4th gRNA with tRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterno promoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-HALO
Plasmid#188943PurposeT7 expression vector for making his6- and HA3-tagged HALO proteinDepositorInserthis6-HA3-HALO
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIVT-myc-T58A-S62D
Plasmid#138540PurposeFor making myc-T58A-S62D RNADepositorInsertMyelocytomatosis oncogene T58A S62D (Myc Mouse)
UseIn vitro transcriptionTagsExpressionMutationThreonine 58 changed to alanine and serine 62 cha…PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-CLIP
Plasmid#188942PurposeT7 expression vector for making his6- and HA3-tagged CLIP proteinDepositorInserthis6-HA3-CLIP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXA2 (murine) HIS-tag pET
Plasmid#8557DepositorInsertHOXA2 (Hoxa2 Mouse)
UseTagsHis and T7ExpressionBacterialMutationcontains 5 additional amino acids from 5' un…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUS
Plasmid#153416PurposeBackbone for cloning downstream of UCOE-SFFVDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXA1 (murine) HIS-tag pET
Plasmid#8556DepositorInsertHOXA1 (Hoxa1 Mouse)
UseTagsHis and T7ExpressionBacterialMutationclone contains 130 AA including the homeodomain, …PromoterAvailable sinceJuly 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAptamerCassette-EGFP
Plasmid#140288PurposeBackbone for making protein-responsive mRNA switchesDepositorInsertEmpty backbone
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMbd3EosHaloTAF_Hyg
Plasmid#140958PurposeTargeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmethyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTa-HH7-2
Plasmid#124997PurposeTo make transgenic mice for HH7-2 TCRaDepositorInsertHH7-2 TCRa Tg
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTa-HH5-1
Plasmid#124995PurposeTo make transgenic mice for HH5-1 TCRaDepositorInsertHH5-1 TCRa Tg
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab2(1-214)
Plasmid#59550Purposemakes 6x Fusion of Full length ceRab2DepositorInsert1-214
UseTagsExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab5(1-208)
Plasmid#59551Purposemakes 6x Fusion of Full length ceRab5DepositorInsert1-208
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab10(1-201)
Plasmid#59552Purposemakes 6x Fusion of Full length ceRab10DepositorInsert1-208
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab11(1-211)
Plasmid#59553Purposemakes 6x Fusion of Full length ceRab11DepositorInsert1-211
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-207)
Plasmid#59554Purposemakes 6x Fusion of Full length ceRab21DepositorInsert1-207
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-ceRab21(1-209)
Plasmid#59555Purposemakes 6x Fusion of Full length ceRab35DepositorInsert1-209
UseTags6x HisExpressionMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-VP64dCas9VP64-2A-MPH
Plasmid#216388Purposethis vector can be used to make modRNA expressing VP64dCas9VP64 and MPHDepositorInsertVP64dCas9VP64-2A-MPH
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-Cas9-2A-p53DD
Plasmid#216389Purposethis vector can be used to make modRNA expressing Cas9 and p53DDDepositorInsertCas9-2A-p53DD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo
Plasmid#153422PurposeMSCV with IRES-Neomycin resistant geneDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pERB215
Plasmid#61499PurposeConst. Halotag-GFP-Nuf2 + mCherry-DHFRDepositorInsertsHaloTag-GFP-Nuf2
mCherry-DHFR
UseTagsHaloTag and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
bactin2:loxP-STOP-loxP-DsRed-express
Plasmid#68797PurposeFor making transgenic fishDepositorInsertsbeta-actin promoter
loxp-stop-codon-loxp-dsred
UseTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
H2B-mGarnet2
Plasmid#104312Purposecodes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cellsDepositorInsertH2B-mGArnet2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSSM110: pCMV-HA-NLS-SpuFz1-v2
Plasmid#205277PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationC310R/D487K/T513KPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B2.0
Plasmid#167158PurposeGolden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-mTagBFP2-N2cG
Plasmid#192838Purposeplasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorInsertmTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
UseAAVTagsExpressionMammalianMutationPromoterTREtightAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-STOP-dsRed
Plasmid#60944PurposeDrosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.DepositorInsertdsRED
UseUnspecifiedTagsExpressionMutationPromoter3XP3Available sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135B2.0
Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSGC-GB1
Plasmid#186786PurposeE. coli expression vector containing N-terminal 6His-GB1-TEV fusion.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-t35s
Plasmid#118385PurposeEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSSM16: pCMV-HA-NLS-SpuFz1
Plasmid#205271PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1DepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p221z-dCAS9p-t35s
Plasmid#118387PurposeEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertdCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
modRNAc1-Cas9-2A-puro
Plasmid#216390Purposethis vector can be used to make modRNA expressing Cas9 and puro resistance geneDepositorInsertCas9-2A-puro
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-stuffer-1397N
Plasmid#187172Purposeoriginal vector to make all constructsDepositorInsertRight-stuffer-1397N
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDZ585 pKAN 2x-mCherry
Plasmid#72236PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherryDepositorInsert2x-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only