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pPHYCUT

Plasmid

#255426

Purpose

Expresses codon-optimized versions of SpCas9 and Csy4, with a U6 promoter-driven BsaI cloning casette for the expression of multiple sgRNAs in Phaeodactylum tricornutum.

Depositor

David Edgell

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Episomal vector for…

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pL0_EF-NR

Plasmid

#202179

Purpose

Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-HO

Plasmid

#202170

Purpose

Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 28, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-NR

Plasmid

#202123

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-HO

Plasmid

#202116

Purpose

Level 0 plasmid containing the promoter from gene 5902 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

HO promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-NR

Plasmid

#202101

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-H4

Plasmid

#202100

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAB184e2

Plasmid

#156362

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Publication

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16, S1, S15 ribosomal genes

Use

Synthetic Biology

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-CtDCP1_X

Plasmid

#148006

Purpose

Bacterial Expression of CtDCP1

Depositor

Elisa Izaurralde

Publication

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP1

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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D3NRV6

Plasmid

#163195

Purpose

Inducible expression of UniProt identifier D3NRV6

Depositor

Martin Engqvist

Publication

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

D3NRV6

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Publication

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Publication

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Expression

Mammalian

Mutation

N/A

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Publication

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

growth arrest specific 8 (Gas8 Mouse)

Tags

PA

Expression

Mammalian

Available since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Publication

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Promoter

EF1α

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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px330-UFSP2 sgRNA2

Plasmid

#134637

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA2 (UFSP2 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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px330-UFSP2 sgRNA1

Plasmid

#134638

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA1 (UFSP2 Human)

Expression

Mammalian

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti PA-NL

Plasmid

#113453

Purpose

Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Publication

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

hUbC

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pONL-N1_LAMP1

Plasmid

#65686

Purpose

Expresses LAMP1-ONL in mammalian cells

Depositor

Yasushi Okada

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

LAMP1

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pNB775

Plasmid

#60115

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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