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pEcCas5

Plasmid

#170090

Purpose

expresses E. coli type I-E Cas5

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas5

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_linker_mKate2

Plasmid

#167335

Purpose

Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJB057

Plasmid

#98911

Purpose

Express Dronpa tagged ZapA in E coli

Depositor

Jie Xiao

Publication

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapA

Tags

Dronpa

Expression

Bacterial

Promoter

T5-lac

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2T

Plasmid

#62258

Purpose

expression of A2T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJIT163-eAFID-3

Plasmid

#167360

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Publication

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3Bctd-Cas9-UDG-T2A-AP lyase

Expression

Plant

Mutation

WT

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

Plasmid

#114111

Purpose

fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Publication

Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

Insert

Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized

Expression

Mammalian

Promoter

CAG

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C3012

Plasmid

#91099

Purpose

Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi1

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pTaCas9_6

Plasmid

#91169

Purpose

Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Expression

Plant

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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Toggle Switch

Plasmid

#251146

Purpose

Toggle Switch (TetR - mCerulean / LacI - GFP)

Depositor

Yolanda Schaerli

Publication

Boni et al eLife15:RP110624

Inserts

LacI
sfGFP
TetR
mCerulean

Expression

Bacterial

Promoter

PLtetO-1 and trc, lacO

Available since

May 26, 2026

Availability

Academic Institutions and Nonprofits only

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EKv413(Gold Cas-guide insert4 2 gene version)

Plasmid

#248342

Purpose

gRNA insert vector for Golden Gate Assembly, 2 gene or 4 gRNAs version

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv414(Gold Cas-guide insert6 3 gene version)

Plasmid

#248343

Purpose

gRNA insert vector for Golden Gate Assembly, 3 gene or 6 gRNAs version

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv376(gRNAscaffold-tRNA for cloning)

Plasmid

#248333

Purpose

Plasmid contains gRNA scaffold-tRNA and is used to amplify fragments for Golden Gate assembly

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Insert

gRNAscaffold-tRNA

Use

CRISPR

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6 - iFAST

Plasmid

#209414

Purpose

iFAST containing plasmid for endogenous fusion constructs

Depositor

Patrick Van Dijck

Publication

Devos et al Microbiology (Reading). 2024 Mar;170(3).

Insert

iFAST

Expression

Yeast

Promoter

none

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDIRECT_22D

Plasmid

#91136

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA v-Src FL

Plasmid

#245255

Purpose

Expression of full-length viral Src in yeast

Depositor

Neel Shah

Publication

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

Full-length viral Src

Expression

Yeast

Mutation

wild-type

Available since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletCm

Plasmid

#206818

Purpose

Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Publication

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAYFPCm

Plasmid

#206826

Purpose

Template for hlpA-YFP amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Publication

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

YFP

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenCm

Plasmid

#206814

Purpose

Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Publication

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Mutation

3insG

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenKm

Plasmid

#206816

Purpose

Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

Depositor

Virginie Libante

Publication

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Available since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSth_Cas6

Plasmid

#178779

Purpose

Expression of S. thermophilus type I-E cas6

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas6

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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