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pTi4.0_SpdCas9_pmcDA1

Plasmid

#204613

Purpose

CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMT85_SpdCas9_pmcDA1

Plasmid

#204614

Purpose

CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b

Plasmid

#215820

Purpose

Express CBE6b (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pRDA_336

Plasmid

#179095

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

BE3.9 (Cas9-NG)

Use

CRISPR and Lentiviral

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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dead Sa Cas9

Plasmid

#138162

Purpose

For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

dSaCas9

Tags

NLS

Expression

Mammalian

Mutation

D10A + N580A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6c-V106W

Plasmid

#215826

Purpose

Express CBE6c V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6c V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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