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We narrowed to 2,726 results for: gem

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Showing: 1501 - 1520 of 2726 results

  1. pMMK ENTR 3 CMV mito mCherry

    Plasmid
    #206256
    Purpose
    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    mito mCherry
    Use
    Multimate/gateway entr 3
    Tags
    COX8 mitochondrial tag
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206256-plasmid-map-sequence-id-406392

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  5. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113953-plasmid-map-sequence-id-215167

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  7. pCEP4-HA-Flag-natT1R2 FS10

    Plasmid
    #113956
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, S212N substitution…
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113956-plasmid-map-sequence-id-215284

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  8. AAV2_hSyn_Phobos_Citrine

    Plasmid
    #98216
    Purpose
    Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98216-plasmid-map-sequence-id-256050

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  10. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

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  11. AAV2_hSyn_Aurora_Citrine

    Plasmid
    #98217
    Purpose
    Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98217-plasmid-map-sequence-id-256049

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  14. pFrt-invCAG-Luc

    Plasmid
    #63577
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63577-plasmid-map-sequence-id-105728

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  17. mCit-Snap25b D179K A199R

    Plasmid
    #53259
    Purpose
    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    D179K and A199R
    Promoter
    CMV
    Available Since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

    Plasmid
    #225442
    Purpose
    BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.
    Depositor
    Diego Orzaez
    Article
    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1
    Insert
    LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225442-plasmid-map-sequence-id-447731

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Showing: 1501 - 1520 of 2726 results