We narrowed to 5,328 results for: pcdna3.1

1. pcDNA3.1 6A CARD8 CARD-FLAG WT

   Plasmid

   #164020

   Purpose

   Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Tras light chain

   Plasmid

   #216294

   Purpose

   Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Pert Light chain

   Plasmid

   #216310

   Purpose

   Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Pert Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Promoter

   T7 promoter

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rab8-T22N(Flag) in pcDNA3.1neo

   Plasmid

   #46784

   Purpose

   expression of T22N Rab8a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB8A T22N (RAB8A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T22N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-Mito-AcGFP-MTn

   Plasmid

   #154350

   Purpose

   Mammalian expression of MTn

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   MTn
   

   Tags

   AcGFP

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-ExRai-AKAR-NES

   Plasmid

   #118407

   Purpose

   Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR-NES
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CMV-mCherry-hPMCA2x/b

   Plasmid

   #111571

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2x/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-OTUB1 T134R-pcDNA3.1

   Plasmid

   #118211

   Purpose

   Expresses FLAG-OTUB1 T134R mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 134 to arginine

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-ERBB4 (kinase dead)

   Plasmid

   #29533

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Expression

   Mammalian

   Mutation

   changed lysine 751 to methionine

   Available Since

   July 20, 2011

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

    Plasmid

    #52502

    Purpose

    expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    deletion of Fbox (deleted aa 71-116)

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTiam1-mcherry-sspB in pcDNA3.1

    Plasmid

    #85221

    Purpose

    Role of Rac selective GEF domain from Tiam1 in immune cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Inserts

    mTiam1 (1030-1406) (Tiam1 Mouse)
    mcherry
    sspB
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/NDUFV1/myc-His

    Plasmid

    #127490

    Purpose

    Expresses myc-tagged NDUFV1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFV1 (NDUFV1 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid

    #194169

    Purpose

    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 V337M
    

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Changed Valine 337 to Methinonine

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CoV2-Omicron BA.1 spike

    Plasmid

    #194602

    Purpose

    Mammalian expression of RBD domain from SARS-CoV-2 omicron (BA.1)

    Depositor

    Ben Murrell

    Article

    Sheward et al Lancet Infect Dis. 2022 Jun;22(6):813-82

    Insert

    CoV2-Omicron BA.1 spike (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

    Plasmid

    #209851

    Purpose

    Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

    Depositor

    Volker Busskamp

    Article

    Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

    Insert

    light-driven sodium pump ErNaR
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-2xFLAG-2xSTREP-CDK2

    Plasmid

    #172616

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10

    Plasmid

    #181859

    Purpose

    Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    cp-PKN-mRuby2-GFP(1-0)
    

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-mCherry-p115(S240A)

    Plasmid

    #181997

    Purpose

    Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115(S240A) (ARHGEF1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Serine 240 changed to alanine in p115.

    Promoter

    CMV

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-SARS2-Spike-D614G

    Plasmid

    #166850

    Purpose

    For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

    Depositor

    Neville Sanjana

    Article

    Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

    Insert

    SARS-CoV-2 Spike (S SARS-CoV-2 virus)
    

    Tags

    C9

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3.1-EGFP(W58stop)-HiBiT

    Plasmid

    #180508

    Purpose

    Mammalian expression of EGFP(W58stop)-HiBiT

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    EGFP(W58stop)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    W58(UGG) to amber stop codon(UAG)

    Promoter

    CMV

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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