We narrowed to 27,336 results for: sta

1. pTH1-SaSrtA-KQ

   Plasmid

   #64982

   Purpose

   Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108Q

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-MGGG-ybbr-HIS-SdrG_B1-DK

   Plasmid

   #117981

   Purpose

   SdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B1
   

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-MGGG-ybbr-HIS-SdrG_B2-DK

   Plasmid

   #117982

   Purpose

   SdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

   Depositor

   Hermann Gaub

   Article

   Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

   Insert

   SdrG_B2
   

   Tags

   HIS, sortase, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR-tagBFP

   Plasmid

   #75160

   Purpose

   Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPR-EGFP

   Plasmid

   #75159

   Purpose

   Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR-RFP657

   Plasmid

   #75162

   Purpose

   Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   RFP657

   Expression

   Mammalian

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-Ls-AChBP

   Plasmid

   #63126

   Purpose

   Expression of Lymnaea stagnalis AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHD157

   Plasmid

   #84033

   Purpose

   Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish

   Depositor

   Didier Stainier, Daniel Hesselson

   Article

   Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

   Inserts

   Cre
   Venus
   

   Use

   Cre/Lox; Zebrafish transgenesis

   Tags

   mCherry

   Promoter

   Crystalin and fabp10

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVk7-33G1-Flag

    Plasmid

    #58950

    Purpose

    backbone for mouse recombinant antibody with added C-terminal FLAG tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVk7-33G1-BioLHis

    Plasmid

    #58949

    Purpose

    backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Tags

    6His tag and biotinylation peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

    Plasmid

    #220224

    Purpose

    AAV-STARR-seq validation control vector with Venus

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCold I-Hero9

    Plasmid

    #187927

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero9

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero9
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBstSK+IRES-V5-IPpoI

    Plasmid

    #206321

    Purpose

    A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

    Depositor

    Brian McStay

    Article

    van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

    Insert

    IRES element-V5 epitope tag- IPpoI open reading frame
    

    Use

    Mrna production

    Tags

    V5-IPpoI fusion protein

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL1-aadA

    Plasmid

    #117050

    Purpose

    In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    aadA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pssAAV_Seq17_CMV_bglobin_3NLS_RFP_WPRE_polyA

    Plasmid

    #220222

    Purpose

    AAV-STARR-seq validation vector with RFP

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA

    Plasmid

    #220221

    Purpose

    AAV-STARR-seq validation vector with GFP

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP-AUA-IRES-mCherry

    Plasmid

    #222116

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AUA start codon

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-AGC-IRES-mCherry

    Plasmid

    #222119

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    AGC start codon

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP-CUG-IRES-mCherry

    Plasmid

    #222110

    Purpose

    Start codon reporter with mutated start codon

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    GFP-IRES-mCherry
    

    Use

    Lentiviral

    Mutation

    CUG start codon

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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