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pJK346

Plasmid

#71836

Purpose

Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-59 to aspartate

Promoter

lac

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-A

Plasmid

#64979

Purpose

Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-K

Plasmid

#64978

Purpose

Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

Plasmid

#117979

Purpose

SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1-B2

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG_N2N3-B1-HIS-ybbr

Plasmid

#117980

Purpose

SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_N2N3-B1

Tags

H3V 3C, HIS, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET28a-MGGG-ybbr-HIS-SdrD_B1-DK

Plasmid

#117983

Purpose

SdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfB

Depositor

Hermann Gaub

Article

Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

Insert

SdrG_B2

Tags

HIS, sortase, and ybbr

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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p3E-FRT-KanR-FRT pA

Plasmid

#82601

Purpose

3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRT-Kan-FRT

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-PSMA

Plasmid

#11524

Depositor

Pamela Bjorkman

Article

Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

Insert

prostate-specific membrane antigen (FOLH1 Human)

Tags

His

Expression

Insect

Mutation

There is a short N-terminal truncation of this pr…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HAI

Plasmid

#71316

Purpose

Inducible expression of human arginase I in E. coli

Depositor

David Christianson

Article

DiCostanzo et al Proc Natl Acad Sci U S A. 2005 Sep 13;10

Insert

human arginase I (ARG1 )

Expression

Bacterial

Promoter

tac

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-DSCP-Gateway

Plasmid

#71507

Purpose

flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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297_pETcon_SARS2_Omicron-BA286

Plasmid

#222231

Purpose

yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYGS Q16C

Plasmid

#217674

Purpose

For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

Depositor

Larry David

Insert

CRYGS (CRYGS Human)

Tags

6XHis and SUMO

Expression

Bacterial

Mutation

Glutamine 16 changed to Cysteine

Promoter

T7

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTS2351_Tier3(SB)-Zeo

Plasmid

#169648

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven ZeoR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJK278

Plasmid

#71134

Purpose

Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide mutase

Expression

Bacterial

Mutation

changes histidine-89 to asparagine; additional N-…

Promoter

T7

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJK130

Plasmid

#71129

Purpose

Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

Depositor

T. J. Kappock

Article

Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

Insert

N5-carboxyaminoimidazole ribonucleotide synthetase

Tags

His6

Expression

Bacterial

Mutation

changed valine-319 to phenylalanine

Promoter

T7

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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