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pAAV_hSyn1_HyPer7_mito

Plasmid

#238968

Purpose

Mammalian neurons HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_SypHer3s_mito

Plasmid

#238972

Purpose

Mammalian neurons SypHer3s expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

SypHer3s

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-SLC66A5_STOP

Plasmid

#221422

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC66A5 (MPDU1 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-MPP1

Plasmid

#221428

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MPP1 (MPP1 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

Plasmid

#210024

Purpose

Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells

Depositor

Vesa Hytönen

Article

Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

Insert

LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003

Expression

Mammalian

Mutation

Encephalomyocarditis virus internal ribosome entr…

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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