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JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156406

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Gateway Cloning

Tags

mScarlet-I

Mutation

mutant KRAS4A-G12V

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

Plasmid

#192784

Purpose

Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Tags

Ig k-chain leader sequence, SNAPf-tag, and mXFPm

Expression

Mammalian

Mutation

mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-MAGT1_STOP

Plasmid

#161468

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MAGT1 (MAGT1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

Plasmid

#156404

Purpose

Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Gateway Cloning

Tags

mTagBFP2

Mutation

mutant KRAS4A-G12D

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2F04

Plasmid

#70901

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

mod(mdg4) (mod(mdg4) Fly)

Use

Gateway Cloning

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1387

Plasmid

#29186

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple118 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1388

Plasmid

#29187

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple119 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1386

Plasmid

#29185

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple117 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1389

Plasmid

#29188

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple120 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1590

Plasmid

#29269

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple119 (HTR1A Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Oct. 21, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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