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sfGFP-Cx43K258stop

Plasmid

#69025

Purpose

Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

super folder GFP

Expression

Mammalian

Mutation

non-monomerized sfGFP fused in frame to N-terminu…

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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alpha1Ddelta-G12-FRB

Plasmid

#87454

Purpose

rapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCs

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

a1D short variants (Cacna1d Rat)

Tags

FRB

Expression

Mammalian

Mutation

delete KYPAKNTTIALQMLERML

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαWT-IRES-EGFP

Plasmid

#183456

Purpose

Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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prNFL-P8R-EGFP

Plasmid

#132598

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-N98S-EGFP

Plasmid

#132600

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-L269P-EGFP

Plasmid

#132601

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

806T>C, 807G>C; Leu269Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P441L-EGFP

Plasmid

#132604

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1322C>T, 1323A>G; Pro441Leu

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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PSD-95_Y12E-pTagRFP

Plasmid

#52672

Purpose

expression of TagRFP-tagged PSD-95_Y12E

Depositor

Johannes Hell

Insert

PSD-95 (Dlg4 Rat)

Tags

tagRFP

Expression

Mammalian

Mutation

Y12E

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pTag5A-rPrmpMyc

Plasmid

#22734

Depositor

Lynn Hudson

Article

Nielsen et al J Neurosci. 2006 Sep 27. 26(39):9881-91.

Insert

Proline-rich transmembrane protein

Tags

Myc

Expression

Mammalian

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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DCX 3UTRm3hp

Plasmid

#28302

Depositor

Joseph Loturco

Article

Bai et al Nat Neurosci. 2003 Dec . 6(12):1277-83.

Insert

Doublecortin 3' Untranslated region mutated

Expression

Mammalian

Mutation

3 base pair mutation in DCX 3'UTR region

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
delta NT delta CT p70S6K1 MSCV puro

Plasmid

#8463

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

delta NT delta CT p70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

p70 s6k1 form

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
Myc-gamma2/deltaILTM4

Plasmid

#119735

Purpose

GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term

Depositor

Angel de Blas

Article

Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3

Insert

GABRG2/GABRD (Gabrg2, Gabrd Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1534 pscAAV mU6 shRNA(PKCd) CMV-IE Nuc-EYFP

Plasmid

#135562

Purpose

An AAV vector expressing shRNA vs rat PKCd and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

shRNA (mouse PKCd)
Nuc-EYFP

Use

AAV and RNAi

Tags

3xNLS

Expression

Mammalian

Promoter

CMV-IE and mU6

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shNts

Plasmid

#132717

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Nts gene which encodes neurotensin

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shNts (Nts Rat)

Use

AAV

Promoter

Synapsin 1

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCRW0.8 p21 (rat)

Plasmid

#16477

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 (Cdkn1a Rat)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
DCX 3UTRhp

Plasmid

#28301

Depositor

Joseph Loturco

Article

Bai et al Nat Neurosci. 2003 Dec . 6(12):1277-83.

Insert

Doublecortin 3' Untranslated region

Expression

Mammalian

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pMT2 JNK3 p46

Plasmid

#12657

Depositor

John Kyriakis

Insert

JNK3 (Mapk10 Rat)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pCMV1-OCT-HA-eGFP

Plasmid

#67480

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Expression

Mammalian

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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