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Plasmid#101844Purposedrives expression of gnd using Ptrc promoterDepositorInsertKan casette followed by ptrc promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
zwf-fbp-opcA-
Plasmid#101839Purposedeletes zwf_fbp_opcA locusDepositorInsertKanamycin casette (in place of synpcc7942_2333-2335 operon)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_EF_syn_BbsI
Plasmid#89921PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_HI_syn_BbsI
Plasmid#89924PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_T_34_ani_trpC
Plasmid#90291PurposeBB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4DepositorInserttrpC
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_L_12_syn_BsaI
Plasmid#89914PurposeLinker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB1_L_34_syn_BsaI
Plasmid#89916PurposeLinker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_AB_syn_BbsI
Plasmid#89917PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_BC_syn_BbsI
Plasmid#89918PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_CD_syn_BbsI
Plasmid#89919PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_DE_syn_BbsI
Plasmid#89920PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_FG_syn_BbsI
Plasmid#89922PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
BB2_L_GH_syn_BbsI
Plasmid#89923PurposeLinker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECT1B
Plasmid#75447PurposePlasmid that serves as the PCR template to create genomic pdt1B insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1B
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1D
Plasmid#75448PurposePlasmid that serves as the PCR template to create genomic pdt1D insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1D
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT1E
Plasmid#75449PurposePlasmid that serves as the PCR template to create genomic pdt1E insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1E
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL
Plasmid#64171Purposeharbours marker genes HIS3 and LEU2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-X4
Plasmid#70140PurposeYeast chr. integration vector with DS3 selection markerDepositorInsertDS3
UseTagsExpressionYeastMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-PqrA
Plasmid#51195DepositorInsertPqrA
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-Venus-cdc25C5
Plasmid#40969DepositorInsertcdc25C5 (CDC25C Human)
UseIn vitro transcriptionTagsVenusExpressionMutationPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNJB99
Plasmid#51521PurposeGateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequenceDepositorInsertZif268:FokI
UseGateway entry vectorTagsNLSExpressionMutationPromoterAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-1ALigN
Plasmid#20024DepositorInsert1A Domain of DNA Ligase N
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) A259C
Plasmid#111329PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site A259CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) C256A
Plasmid#111331PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationbranch site C256APromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/tdTomato-nat
Plasmid#120326PurposeBacterial tdTomato labelingDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterproDAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMOD3-mClover-Sh_ble
Plasmid#120329PurposeBacterial mClover promoter trapDepositorInsertmClover
UseTagsExpressionBacterialMutationD103N (see comment below)Promoter(None)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pAKW.119-R5-TEV-SUMO-His
Plasmid#154050PurposeExpresses R5 peptide in E. coliDepositorInsertSilaffin R5 peptide
UseTagsSUMO and His tagExpressionBacterialMutationPromoterT5LacOAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Sp
Plasmid#174726PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Pol-II WT
Plasmid#35175DepositorInsertRPBI (POLR2A Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-BMLF1
Plasmid#72636PurposeMammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulatorDepositorInsertBMLF1/BSLF2
UseTagsExpressionMammalianMutationQ157HPromoterAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-gRNA
Plasmid#226867PurposeLentiviral expression of Sp-gRNA with BlpI and BstXI restriction sites for gRNA spacer cloning. Also expresses BFP.DepositorInsertLenti Sp-gRNA
UseLentiviralTagsExpressionMutationPromotermU6Available sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMM-GCaMP6f
Plasmid#127874PurposeFluorescent reporter for mitochondrial outer membrane Ca2+DepositorInsertOuter membrane localized GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pm-iATPSnFR1.0
Plasmid#102548Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP11-His3MX
Plasmid#91951PurposeUniversal C-terminal GFP11 taggingDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL0_24 [pBBR1-UP ORI]
Plasmid#198937PurposeLevel 0 partDepositorInsertpBBR1-UP ORI
UseSynthetic BiologyTagsExpressionMutationS100L (C299T)PromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pm-iATPSnFR1.1
Plasmid#102549Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Cm
Plasmid#174725PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
FlucWT-HA-GFP11-N1
Plasmid#91954PurposeExpresses FlucWT-HA-GFP11 in mammalian cellsDepositorInsertFlucWT-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tol2
Plasmid#31823DepositorInsertTol2 Transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCitrine-pBAD
Plasmid#54723PurposeLocalization: Protein Expression Vector , Excitation: 516, Emission: 529DepositorTypeEmpty backboneUseTags6xHis and mCitrineExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 ermB cassette
Plasmid#117121PurposeErythromycin resistance cassetteDepositorInsertErythromycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
cyto-iATPSnFR1.0
Plasmid#102550Purposecytoplasmic ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.0
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 kanR cassette
Plasmid#117122PurposeKanamycin resistance cassetteDepositorInsertKanamycin resistance cassette for Bacillus subtilis selection
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.1
Plasmid#102547Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLGB13
Plasmid#126618PurposeSuicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 - hSRSF6
Plasmid#120419Purposeoverexpression of human SRSF6DepositorInsertSRSF6 (SRSF6 Human)
UseTagsExpressionMammalianMutationPromoterCMV immediate/earlyAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKTrfp
Plasmid#59772PurposepKT230 derivative; PBAD_rfp; cmRDepositorInsertrfp
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only