We narrowed to 4,045 results for: golden gate
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Plasmid#195729PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#15 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F17
Plasmid#195731PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#17 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F19
Plasmid#195733PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#19 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F2
Plasmid#195716PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#2 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F11
Plasmid#195725PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#11 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F16
Plasmid#195730PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#16 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F18
Plasmid#195732PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#18 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F1
Plasmid#195715PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#1 of a 24 fragment split in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F9
Plasmid#195723PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#9 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F23
Plasmid#195737PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#23 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-mini v2-HF24_F21
Plasmid#195735PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#21 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyAvailable SinceFeb. 23, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ131-RZ-Cas12j2
Plasmid#189782PurposeGolden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-RZ-Cas12j2
Plasmid#189783PurposeGolden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-RZ-Cas12j2
Plasmid#189785PurposeGolden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB61 - pL0_V2 (CDS1)
Plasmid#123183PurposeGolden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005DepositorInsertTomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)
UsePart for plant expressionExpressionPlantMutationNo BpiI and BsaI sitesAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_SRDX
Plasmid#136976PurposeGoldengate/Greengate module EAR-repression domainDepositorInsertSRDX
ExpressionBacterialAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_mScarlet
Plasmid#136967PurposeGoldengate/Greengate CDS module, containing mScarletDepositorInsertmScarlet
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_Tq2CFP
Plasmid#136969PurposeGoldengate/Greengate CDS module, containing Tq2CFP.DepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_2NLS
Plasmid#136977PurposeGoldengate/Greengate module nuclear localization signalDepositorInsertSV40 NLS
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGB_RT_Tq2CFP_li
Plasmid#136965PurposeGoldengate/Greengate N-tag module, containing Tq2CFP fused to a flexible linkerDepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGA_RT_pLexA
Plasmid#136961PurposeGoldengate/Greengate module for generation of beta-estradiole inducible genes.DepositorInsertpLexA
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_li_Tq2CFP
Plasmid#136971PurposeGoldengate/Greengate C-tag module, containing Tq2CFP fused to a flexible linkerDepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGB_RT_Venus_li
Plasmid#136966PurposeGoldengate/Greengate N-tag module, containing Venus fused to a flexible linkerDepositorInsertVenus
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGB_RT_tdTomato_li
Plasmid#136964PurposeGoldengate/Greengate N-tag module, containing tdTomato fused to a flexible linkerDepositorInserttandem Tomato
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_Sa_sgRNA
Plasmid#136968PurposeGoldengate/Greengate module for genome editing, using the Sa guideRNA.DepositorInsertSa guide RNA
UseCRISPRAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGI_RT_Sa_sgRNA
Plasmid#136982PurposeGoldengate/Greengate module for genome editing, using the Sa guideRNA.DepositorInsertSa guide RNA
UseCRISPRAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_Sa_sgRNA
Plasmid#136975PurposeGoldengate/Greengate module for genome editing, using the Sa guideRNA.DepositorInsertSa guide RNA
UseCRISPRAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_li_Tq2CFP_2NLS
Plasmid#136972PurposeGoldengate/Greengate C-tag module, containing Tq2CFP fused to 2 SV40 NLS and a flexible linkerDepositorInsertTq2CFP
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGE_RT_Sa_sgRNA
Plasmid#136978PurposeGoldengate/Greengate module for genome editing, using the Sa guideRNA.DepositorInsertSa guide RNA
UseCRISPRAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_XVE
Plasmid#136970PurposeGoldengate/Greengate module for generation of beta-estradiole inducible genes.DepositorInsertXVE
ExpressionBacterialMutationBsaI site domestication 834 T > A,Available SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGA_RT_Sa_Cas9
Plasmid#136962PurposeGoldengate/Greengate module for genome editing, using the SaCAS9.DepositorInsertSaCAS9
UseCRISPRAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGD_RT_biChromium
Plasmid#136974PurposeGoldengate/Greengate C-tag module, containing a dual fluorescent marker based on the P2A peptideDepositorInsertP2A sequence
ExpressionBacterialAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Tq2CFP
Plasmid#136981PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGGF_RT_FAST_Venus
Plasmid#136980PurposeGoldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescenceDepositorInsertseed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
ExpressionBacterialMutationBsaI site domestication in pOLE1:OLE1-RFP 688 G &…Available SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKMW123_SpRY-Cas9_U6-entry
Plasmid#244820PurposeMammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassetteDepositorInsertSpRY Cas9
UseCRISPRTags3xFLAG-SV40 NLS and Nucleoplasmin NLSExpressionMammalianMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterCAG, U6Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459v3
Plasmid#178799PurposeSpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv4
Plasmid#221432PurposeLentiviral CRISPR/Cas9 vector for co-expression of sgRNAs and Cas9 protein.DepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW411d
Plasmid#246585Purposedifferent combination of A. thaliana and N. tabacum Rubisco assembly factorsDepositorInsertExpressionBacterialAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-SRSF1 siRNA-resitant
Plasmid#218974PurposeVector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)DepositorInsertSRSF1 cDNA
ExpressionMammalianMutationsiRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…PromoterCMVAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
Plasmid#229545PurposeLentiviral construct to express rtTA and SKM proteinsDepositorInsertrTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
UseLentiviralExpressionMammalianPromoterhEF1aAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esmGFP-C10-pVTCB052
Plasmid#231303PurposePlasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coliDepositorInsertesmGFP-C10
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9569
Plasmid#219431PurposeTranscriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOSDepositorInsertLB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsPapE-4LF2ExpressionPlantAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
Plasmid#192822PurposeGolden Gate compatible plasmid with mCherry and NeoDepositorInsertmCherry and Neo
ExpressionMammalianAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2
Plasmid#246051PurposeExpression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12aDepositorInsertAcrVA1_cpGR2-S103ΔELS
UseCRISPR and Synthetic BiologyExpressionMammalianMutationΔELSAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-YB_TATA)-mCherry-HRasG12V-BGH_pKG2744
Plasmid#246340Purpose203-bp DIAL Reporter Plasmid with YB_TATA expressing mCherry-GS-HRasG12V in the presence of ZFa and editable by Cre recombinaseDepositorAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW334e3
Plasmid#246588Purposesequential knock out of A. thaliana Rubisco assembly factorsDepositorInsertExpressionBacterialAvailable SinceNov. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW411c
Plasmid#246584Purposedifferent combination of A. thaliana and N. tabacum Rubisco assembly factorsDepositorInsertExpressionBacterialAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW411a
Plasmid#246582Purposedifferent combination of A. thaliana and N. tabacum Rubisco assembly factorsDepositorInsertExpressionBacterialAvailable SinceOct. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-H505A-H741A-H975A
Plasmid#238337PurposeproUBI10::VIH2-FL::FLAG-H505A-H741A-H975A ubiquitous expression in Arabidopsis plantsDepositorAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only