We narrowed to 2,484 results for: NIG

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pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2)

Plasmid

#223789

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Mutation

E72A/L75A/D77A/E81A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI4

Plasmid

#223769

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI4 (WDR45 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-ATG13 IDR (191-517aa)

Plasmid

#223762

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

ATG13 IDR (ATG13 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

Plasmid

#223766

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

W18A/L21A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

Plasmid

#223738

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

W36A/L39A; deletion of LIR

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST

Plasmid

#223745

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BCL2L13 soluble part (BCL2L13 Human)

Tags

EGFP-TEV-GST

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT&Mut-SM

Plasmid

#215916

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT-SM

Plasmid

#215917

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut1-SM

Plasmid

#215918

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_Mut2-SM

Plasmid

#215919

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT&Mut-CM

Plasmid

#215920

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_WT&Mut (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-BRAF_WT-CM

Plasmid

#215921

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ WT (BRAF Human)

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-BRAF_Mut1-CM

Plasmid

#215922

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut1 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-BRAF_Mut2-CM

Plasmid

#215923

Purpose

This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

Insert

one complete-matched (CM) sequence of siBRAF_ Mut2 (BRAF Human)

Expression

Mammalian

Mutation

BRAF

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast 3xFlag-SPNS1(R76A)

Plasmid

#198570

Purpose

SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

changed arginine R76 to alanine (R76A)

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-IRES-blast GFP-SPNS1(E164K)

Plasmid

#198572

Purpose

SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

changed glutamic acid E164 to lysine (E164K)

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(K289N).ires.GFP-pcDNA3

Plasmid

#204364

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

K289N

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(P369T).ires.GFP-pcDNA3

Plasmid

#204365

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

P369T

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(R443Q).ires.GFP-pcDNA3

Plasmid

#204366

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Mutation

R443Q

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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