We narrowed to 3,078 results for: cat.3

1. Gfp-rt-vasa

   Plasmid

   #167322

   Purpose

   Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

   Depositor

   Goro Yoshizaki

   Article

   Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

   Insert

   rt vasa 3'UTRs (ddx4 rainbow trout)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBM14_pLX307

   Plasmid

   #98367

   Purpose

   Lentiviral expression of RBM14

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   RBM14 (RBM14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-HADH2

   Plasmid

   #67863

   Purpose

   bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

   Depositor

   Walter Rossmanith

   Article

   Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

   Insert

   HADH2 (HSD17B10 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-Puro-NCOA7g1 (BB22)

   Plasmid

   #139457

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-Puro-NCOA7g2 (BB23)

   Plasmid

   #139458

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

   Plasmid

   #176061

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

   Plasmid

   #175423

   Purpose

   Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 1-117 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   nsp1 C-terminus

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA4 3xFLAG Halo delta 118-180 nsp1

   Plasmid

   #175424

   Purpose

   Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 delta 118-180 nsp1
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   N-terminus of nsp1

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MRPP3-myc-His

    Plasmid

    #67864

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His and myc

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 RL3m6L2

    Plasmid

    #109366

    Purpose

    Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rb-379-928Δ703-737-pGex2T

    Plasmid

    #21933

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928D703-737 o…

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3b(1454/3172)P2P-mut

    Plasmid

    #21405

    Depositor

    Eric Metzen

    Article

    Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

    Insert

    EGLN1 (EGLN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    hypoxia response element mutated

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

15. Rb-379-928Δ738-775-pGex2T

    Plasmid

    #21783

    Depositor

    William Kaelin

    Article

    Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion mutant ORF contains aa 379-928D737-774 o…

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGADT7-ε

    Plasmid

    #197261

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-4 ε (AP4E1 Human)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    silent substitutions in codons 905, 1129 and 1137

    Promoter

    ADH1

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4 3xFLAG Halo G-Linker nsp1

    Plasmid

    #175426

    Purpose

    Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    G-linker nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    G-linker in place of the RNase destabilization do…

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA4 3xFLAG Halo R119A K120A nsp1

    Plasmid

    #175428

    Purpose

    Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 R119A K120A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants R119A K120A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA4 3xFLAG Halo E36A E37A nsp1

    Plasmid

    #175429

    Purpose

    Expresses E36A E37A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 E36A E37A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants E36A E37A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDTCendoNA2

    Plasmid

    #174732

    Purpose

    Expresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linker

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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