We narrowed to 2,514 results for: organization Addgene

1. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

   Plasmid

   #159455

   Purpose

   Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

   Depositor

   Oliver Griesbeck

   Article

   Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

   Insert

   mCRISPRed
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Calreticulin-SuperNova-KDEL

   Plasmid

   #131857

   Purpose

   Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Use

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZJOM160

   Plasmid

   #133670

   Purpose

   Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-mTagBFP2-SKL
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPRv2-TRAF6-targeting sgRNA 1

   Plasmid

   #131345

   Purpose

   gRNA targeting mouse TRAF6

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   gRNA targeting mouse TRAF6 (Traf6 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM144

   Plasmid

   #133630

   Purpose

   Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-GFP-HDEL
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMB-TMEr

   Plasmid

   #61208

   Purpose

   Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   MtBCPsp-mCherry-BCP
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.nucGAP

   Plasmid

   #78736

   Purpose

   Expression of Ca2+ sensor GAP in the nucleus

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   nucGAP
   

   Use

   Tags

   nucleoplasmin (Xenopus)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pGrDL_SP

   Plasmid

   #83204

   Purpose

   receives miRNA target sequences for testing using the dual luciferase assay system

   Depositor

   Peer Schenk

   Article

   Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

   Insert

   SalI/PstI cloning sites
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2-TRAF6-targeting sgRNA 2

   Plasmid

   #131346

   Purpose

   gRNA targeting mouse TRAF6

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   gRNA targeting mouse TRAF6 (Traf6 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    longCaMV35S

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-GFP-3xFLAG-MyD88-V5

    Plasmid

    #131351

    Purpose

    Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88 (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMB-NUCr

    Plasmid

    #61169

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUF-AsCpf1-pre-gRNA

    Plasmid

    #137849

    Purpose

    AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    AsCpf1 and pre-gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUF-LbCpf1-pre-gRNA

    Plasmid

    #137848

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG and gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. NPC1-CFP P692S

    Plasmid

    #58457

    Purpose

    expression of NPC1-CFP sterol sensing domain mutant

    Depositor

    Matthew Scott

    Article

    Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

    Insert

    NPC1 (Npc1 Mouse)
    

    Use

    Tags

    ECFP and His6

    Expression

    Mammalian

    Mutation

    P692S sterol sensing domain mutant

    Promoter

    CMV

    Available since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pLV 4xSTAR-mTFP-NLS-blast

    Plasmid

    #136257

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTFP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM41-EcPPXc

    Plasmid

    #38329

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pMSCV-IRES-GFP-MyD88-NpLxIS

    Plasmid

    #131347

    Purpose

    Expresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    MyD88-NpLxIS (Myd88 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Use

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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