We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1(+)Gαq-124-NLuc

   Plasmid

   #207153

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAQ (GNAQ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(124)E

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

   Plasmid

   #207160

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

   Plasmid

   #127322

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Synthetic, Human)
   

   Use

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RGS-6xHis-mRFP-pcDNA3.1-

   Plasmid

   #52516

   Purpose

   expresses mRFP in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   mRFP
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   engineered variant of tetrameric red fluorescent …

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

   Plasmid

   #50031

   Purpose

   Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   PM-SrtA(strep)
   

   Use

   Tags

   C-HA

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP Rab11a WT pcDNA3.1 Zeo (+)

   Plasmid

   #206079

   Purpose

   Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

   Depositor

   Annette Dolphin

   Article

   Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

   Insert

   RAB11A (RAB11A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Hygro-Mfn2-ActA

   Plasmid

   #191981

   Purpose

   Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

   Depositor

   Luca Scorrano

   Insert

   Mfn2-ActA
   

   Use

   Tags

   ActA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EphA2 430-976 pcDNA3.1

   Plasmid

   #102738

   Purpose

   Expression of c terminal EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

   Insert

   EphA2 N terminal (430-976aa) (Epha2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-MS2-HA-HsRoquin1_1-509_AE

    Plasmid

    #148612

    Purpose

    Mammalian Expression of HsRoquin1_1-509

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_1-509 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MS2-HA-HsRoquin1_510-1133_AE

    Plasmid

    #148613

    Purpose

    Mammalian Expression of HsRoquin1_510-1133

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin1_510-1133 (RC3H1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) neo with Smad5 CDS

    Plasmid

    #61800

    Purpose

    pcDNA3.1(+) with full length mouse Smad5 CDS

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    Smad5 CDS (Smad5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1-Hyg-mEGFP-pp4640

    Plasmid

    #191846

    Purpose

    Expresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusion

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP-pp4640
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    mEGFP fusion in Nterminal, some silent point muta…

    Promoter

    CMV

    Available since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 AT1R DRY-AAY

    Plasmid

    #45759

    Depositor

    Laszlo Hunyady, Robert Lefkowitz

    Article

    Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

    Insert

    AT1R DRY-AAY (Agtr1a Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed amino acids 125-126 from DR to AA

    Promoter

    CMV

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-2xFLAG-2xSTREP-CCND3

    Plasmid

    #172623

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 myc his Su(fu) (CT#768)

    Plasmid

    #13855

    Depositor

    Cliff Tabin

    Article

    Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

    Insert

    Su(fu) (SUFU Chicken)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    full ORF

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-HA-FbxO11-Jeff-pcDNA3.1-

    Plasmid

    #52503

    Purpose

    expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO11 (FBXO11 Human)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Q491L (Jeff)

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Flag-ZFP809(1-353)

    Plasmid

    #67788

    Purpose

    Mammalian expression of 1-353 amino acids of ZFP809 tagged with Flag

    Depositor

    Stephen Goff

    Article

    Wolf et al Nature. 2009 Apr 30;458(7242):1201-4. do

    Insert

    ZFP809 (Zfp809 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Cry2 mCherry PGK-1 in pcDNA3.1

    Plasmid

    #64214

    Purpose

    mCherry fused between Cry2 and PGK-1 to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 PHR (CRY2 Mustard Weed)
    PGK1 (PGK1 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1-2xFLAG-2xSTREP-CCND2

    Plasmid

    #172629

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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