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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA178

Plasmid

#216046

Purpose

Fragmid fragment: (C' terminus) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (hSyn-Cre) mTubb3

Plasmid

#200369

Purpose

Positive control plasmid expressing mouse Tubb3 GS-gRNA (with hSyn-Cre)

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Mouse Tubb3 gRNA and Cre

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

Plasmid

#233079

Purpose

Expression vector for sgRNA-S1b and eCas12f1a

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Promoter

hU6

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

Plasmid

#156449

Purpose

For transient expression of human PDS5A tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5A (PDS5A Human)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

Plasmid

#92140

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-HSPB8

Plasmid

#34710

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

heat shock 22 kDa protein 8 (HSPB8 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pNP2987 [pNP2922 - ISCro4 TBL3 (WT) + ISCro4 Recombinase]

Plasmid

#247256

Purpose

Express ISCro4 recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2922 - ISCro4 bRNA35-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL3)

Expression

Mammalian

Mutation

C60U, A61G, U73C, dU86, A87G, dU88

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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