We narrowed to 6,180 results for: cas9 expression plasmid
-
Plasmid#80938PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-POLI-ST2-com vector
Plasmid#176234PurposeVector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replaceDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42229PurposeThis plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)
Plasmid#223079PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)
Plasmid#223087PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)
Plasmid#223094PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)
Plasmid#223075PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)
Plasmid#223068PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(MRKCRS)-P2A-EGFP (RAS2821)
Plasmid#223102PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCRS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(MRKCRS)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(MRKCRS)=D1135M, S1136R, G1218K, E1219C, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9(SRQMRG)-P2A-EGFP (RAS3017)
Plasmid#223103PurposepCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRQMRG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized nuclease SpCas9(SRQMRG)-P2A-EGFP
UseCRISPRTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpCas9(SRQMRG)=D1135S, S1136R, G1218Q, E1219M, R1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only