We narrowed to 1,094 results for: gibson assembly
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Plasmid#222099PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.DepositorInsertVitiosangium-bGSDM-thrombin ORF
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Nostoc-bGSDM-WELQ
Plasmid#222100PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.DepositorInsertNostoc-bGSDM-WELQ ORF
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK301
Plasmid#110593PurposeBTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)DepositorInsertBba_B0015 Terminator
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-SuperNovaGreen-T2A-dTomato
Plasmid#193798PurposeExpresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomatoDepositorInsertSuperNova Green
UseAAVTagsFlag tagExpressionMammalianPromoterCAGAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOPO tTR-KRAB-rtTA3-Luc
Plasmid#68449PurposeTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible geneDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseLuciferase; GmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
TagsHaloTagExpressionMammalianMutationDeletion of lumenal domainAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3762 : CMVd3-HsIRE1(K121Y)-HaloTag
Plasmid#185684PurposeLow-level transient expression of IF1 mutant HsIRE1 with a C-terminal HaloTag.DepositorAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-K2K1
Plasmid#242820PurposeEncodes human kindlin2 containing kindlin1 F3 domain and N-terminal EBFP2 tagDepositorInsertKindlin2 (FERMT2 Human)
ExpressionMammalianMutationKindlin2 chimera containing the F3 domain of kind…Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Integrin β6β1-EGFP
Plasmid#242829PurposeEncodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tagDepositorInsertIntegrin subunit beta 6, transcript variant 1 (ITGB6 Human)
ExpressionMammalianMutationIntegrin β6 chimera containing the cytoplasmic ta…Available SinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only