We narrowed to 2,376 results for: pet28
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Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
Tags6His-TEVExpressionBacterialMutationP51APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R52A)
Plasmid#112729Purpose52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.DepositorInsert6His-TEV-TBP6.7(R52A)
Tags6His-TEVExpressionBacterialMutationR52APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
Tags6His-TEVExpressionBacterialMutationQ54APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_T1-NHis
Plasmid#109259PurposeNRPS T domain with N-terminal 6His tagDepositorArticleInsertRupA_T1
TagsHis tagExpressionBacterialMutationcontains aa 917..1025 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RupA_R-NHis
Plasmid#109257PurposeNRPS R domain with N-terminal 6His tagDepositorArticleInsertRupA_R
TagsHis tagExpressionBacterialMutationcontains aa 2038..2443 of WP_015523502.1PromoterT7Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-Metavinculin tail
Plasmid#107152Purposebacterial expressionDepositorAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd5_41-192/pET28b(+)
Plasmid#83296Purposecsd5, construct: 41-192, pET28b(+) N-tagDepositorInserthp1250
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-40Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd4_22-438/pET28b(+)
Plasmid#83295Purposecsd4, construct: 22-438, pET28b(+) N-tagDepositorInserthp1075
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-21Available SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only