We narrowed to 8,597 results for: App

1. pEMY01CD-TER

   Plasmid

   #127751

   Purpose

   Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCellFree_G06_ZIKV NS2B

   Plasmid

   #145354

   Purpose

   Cell-free expression vector for Zika virus NS2B protein with C-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS2B
   

   Use

   Tags

   mCherry and 8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmCellFree_KA2_ZIKV NS1

   Plasmid

   #145362

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS1 protein with N-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS1
   

   Use

   Tags

   8xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmCellFree_KA2_ZIKV NS3

   Plasmid

   #145365

   Purpose

   Cell-free/mammalian expression vector for Zika virus NS3 protein with N-term mCherry

   Depositor

   Rob Parton

   Article

   Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

   Insert

   ZIKV NS3
   

   Use

   Tags

   8xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTetO-hOSK-CASH-1

   Plasmid

   #188977

   Purpose

   A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   Doxycycline inducible reprogramming donor cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX303-ZIM3-KRAB-dCas9

   Plasmid

   #154472

   Purpose

   Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. V5-LOV-Turbo1-NIK3x_pTRE-G418

   Plasmid

   #202017

   Purpose

   expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-LOV-Turbo1-NIK3x
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-Tight

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Snap-Omp25

   Plasmid

   #69599

   Purpose

   Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   Snap-Omp25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. bact2-mKate2-P2A-TurboID-dVHH[GFP]

   Plasmid

   #163857

   Purpose

   Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID-VHH[EGFP]
   

   Use

   Zebrafish expression

   Tags

   mKate2-P2A-TurboID

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. STAGR_gRNAScaffold_mU6

    Plasmid

    #102844

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert gRNAScaffold_mU6
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. STAGR_gRNAScaffold_hH1

    Plasmid

    #102841

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    STAgR Insert gRNAScaffold_hH1
    

    Use

    Pcr template for stagr inserts

    Tags

    Expression

    Mutation

    Promoter

    hH1

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. acta1-Cavin4a-Clover

    Plasmid

    #163853

    Purpose

    Zebrafish muscle specific expression of Cavin4a-Clover, Parton lab clone DPV

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Cavin4a (cavin4a Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    Clover

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

    Plasmid

    #126679

    Purpose

    Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

    Depositor

    Hee Won Yang

    Article

    Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

    Insert

    Rb (a.a 886-928)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby

    Plasmid

    #71760

    Purpose

    mGFP and Synaptophysin-mRuby expression from Cre-expressing neurons

    Depositor

    Liqun Luo

    Article

    Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

    Has Service

    AAV1

    Inserts

    mGFP
    Synaptophysin
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitoylation signal and mRuby

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TRE-KRAB-dCas9-IRES-BFP

    Plasmid

    #85449

    Purpose

    Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLPP6

    Plasmid

    #209968

    Purpose

    Contains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertion

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter Dropout (sfGFP)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)

    Plasmid

    #110572

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD-stRBS-aacC1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTn7CdrA-gfp(ASV)C

    Plasmid

    #111617

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLD-puro-2A-rtTA-TcVA

    Plasmid

    #24592

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTn7CdrA-gfpC

    Plasmid

    #111616

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(Mut3)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. iRFP-Sec61beta

    Plasmid

    #108125

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    iRFP:SEC61B (SEC61B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FEMP

    Plasmid

    #191973

    Purpose

    FRET-based indicator of ER-mitochondria proximity (FEMP)

    Depositor

    Luca Scorrano

    Article

    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    AkapYFP-Tav2a-CFP-sac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. V5-LOV-Turbo1-G3BP1_pTRE-G418

    Plasmid

    #202015

    Purpose

    expresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-LOV-Turbo1-G3BP1
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-Tight

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-Dcp1a

    Plasmid

    #153972

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1a

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA decapping enzyme 1a (DCP1A Human)
    

    Use

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-CRY2-mCherry

    Plasmid

    #58368

    Purpose

    Mamlian codon optimized CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. L_NLS 41kDa

    Plasmid

    #201342

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p15a_Kan_MLS_Rosa26_Short_CRISPRDel

    Plasmid

    #97008

    Purpose

    Rosa26 locus targeting vector

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GFP-Foxo1

    Plasmid

    #17551

    Depositor

    Domenico Accili

    Article

    Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

    Insert

    pEGFP-N1 Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMD2-VSVGmut

    Plasmid

    #182229

    Purpose

    Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    VSVGmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MS2-HB

    Plasmid

    #35573

    Depositor

    Marian Waterman

    Article

    Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

    Insert

    MS2 Coat Protein (cp Bacteriophage)
    

    Use

    Retroviral

    Tags

    HTBH

    Expression

    Mammalian

    Mutation

    Insert is a tail-to-head tandem placement of two …

    Promoter

    CMV

    Available since

    May 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-HA-JMJD3

    Plasmid

    #24167

    Purpose

    Mammalian expression of human JMJD3 with HA tag

    Depositor

    Kristian Helin

    Article

    Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ChromosomesMembranesandCytoskeleton

    Plasmid

    #62450

    Purpose

    MXS_chaining vector with 4 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::H2B-TagBFPx3-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::mCherryx3-linker-betaActin-bGHpA
    CMV::Citrinex3-linker-alphaTubulin-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-CCR5

    Plasmid

    #98943

    Purpose

    Mammalian expression plasmid for untagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLJM1 Raptor GFP

    Plasmid

    #112744

    Purpose

    express Raptor-GFP

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Raptor-GFP (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. 6xHis-SNAP-PI(3,4)P2 Probe

    Plasmid

    #211511

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    TAPP1 cPHx3
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. M_NLS 41kDa

    Plasmid

    #201341

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A5-EGFP-2PrA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLentiCRISPR-EGFP

    Plasmid

    #75159

    Purpose

    Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. EF1a_mCherry_P2A_Hygro

    Plasmid

    #135003

    Purpose

    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. TL026_AAV_hSyn:SEP-GluA1

    Plasmid

    #192519

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    AAV and Cre/Lox

    Tags

    super ecliptic phluorin

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLR16-Phes*

    Plasmid

    #98783

    Purpose

    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

    Depositor

    Anat Herskovits

    Article

    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

    Insert

    pheS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A309G

    Promoter

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pUC19/mChe-Golgi

    Plasmid

    #183169

    Purpose

    Construct for transient expression of Golgi apparatus-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Golgi
    

    Use

    Tags

    AtRER1B, A. thaliana endoplasmic reticulum retrie…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pEHAC1-CHIP-BirA-HA

    Plasmid

    #232587

    Purpose

    Express BirA tagged to the C-terminus of CHIP.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    CHIP - BirA (CHIP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSBinit

    Plasmid

    #110100

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTag

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    myc, His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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