We narrowed to 2,923 results for: COB;

1. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS305-Leu2-PINO4-PfV-GS-Sapphire

   Plasmid

   #116930

   Purpose

   Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   INO4

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

   Plasmid

   #116931

   Purpose

   Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   lumazine synthase
   

   Use

   Tags

   Sapphire

   Expression

   Yeast

   Mutation

   Promoter

   HIS3

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFB2B

   Plasmid

   #195284

   Purpose

   Bicistronic HSV-TK and Puromycin resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A Puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSPIH6

   Plasmid

   #190676

   Purpose

   E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

   Depositor

   John Vederas

   Article

   Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

   Inserts

   Small ubiquitin-like modifier
   Mxe GyrA intein with C-terminal chitin binding domain and His tag
   

   Use

   Tags

   Chitin binding domain, His tag, and N-terminal Hi…

   Expression

   Bacterial

   Mutation

   Added C-terminal his tag and None

   Promoter

   T7

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dCas9-EGFP

   Plasmid

   #232761

   Purpose

   Expresses dCas9-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFB2F

   Plasmid

   #195289

   Purpose

   Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A mScarlet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBeta-lacZ-attBx2

   Plasmid

   #124445

   Purpose

   Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

   Depositor

   Yingguang Frank Chan

   Article

   Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

   Type

   Empty backbone

   Use

   Enhancer reporter assay construct

   Tags

   LacZ

   Expression

   Mammalian

   Mutation

   Promoter

   beta globin minimal promoter

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFB2C

    Plasmid

    #195285

    Purpose

    Bicistronic HSV-TK and Hygromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-N1_MeCP2(WT)

    Plasmid

    #110186

    Purpose

    Expression of mouse MeCP2 (WT) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas9-dMSK1

    Plasmid

    #165602

    Purpose

    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Synthetic, S. Pyogenes, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKRG3-mU6-PUb-hSpCas9-pAc

    Plasmid

    #162163

    Purpose

    Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    T2A-pAc

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFB2G

    Plasmid

    #195290

    Purpose

    Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A mTagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti CMV SNAP MDC1

    Plasmid

    #227375

    Purpose

    SNAP–MDC1 expression

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS306-URA3-PHIS3-PfV-GS-Sapphire

    Plasmid

    #116932

    Purpose

    Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmIL-6promoterEGFP

    Plasmid

    #112896

    Purpose

    To monitor the activation of the murine IL-6 promoter by eGFP expression

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    IL-6 promoter

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINB-hRNF216

    Plasmid

    #167354

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge