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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 46 results for: COB;

Showing: 1 - 20 of 46 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to their... “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA...efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune’s lab screened 11 other... 32:1141–1145 . https://doi.org/10.1038/nbt.3011 Cobb RE, Wang Y, Zhao H (2014) High-Efficiency Multiplex...2016) Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference...

  2. Bacterial Expression Systems

    Type
    Collection
    ...coli , Mycobacterium smegmatis Carolyn Bertozzi , Jessica Seeliger 202406 pJYP1 Riboswitch Cobalamin (vitamin...Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Sabine Ehrt 44561 pST-KT Pmyc1/...Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Vinay Nandicoori 78577 pL99 PnitA-NitR...Acetamidase promoter Acetamide Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 84692 pMyC-kan Acetamidase...Acetamidase promoter Acetamide Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 84689 pMyBADC-kan...pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 17806 pPro18 pPrpB Propionate...pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis Matthias Wilmanns 44447 pLC290 pR/...

  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make... project can be a dangerous thing. Fortunately, Jacob was able to quickly explain that it was common knowledge...conformation that was not “poised for cleavage”. Jacob computationally added the non-template strand to...an effective teaching tool. You can read about Jacob’s initial reaction to this model on his blog. Or,...

  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Gray) and two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging...10.1016/j.cell.2013.01.038 Guy J, Gan J, Selfridge J, Cobb S, Bird A (2007) Reversal of Neurological Defects...Gadalla KKE, Heindel C, Chen D, Zaric V, Bailey MES, Cobb SR, Gray SJ (2017) Improved MECP2 Gene Therapy Extends...Koerner MV, Gadalla KKE, Guy J, De Sousa D, Hector RD, Cobb SR, Bird A (2017) Radically truncated MeCP2 rescues...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...10.1093/genetics/iyae126.   Gene disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system...dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly common human pathogen...-based systems have been developed for other mycobacteria, but such systems don’t always work across species...for rapid and efficient genetic disruption in Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https...

  7. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide (PZA...methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies ...

  8. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...The following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science...among our members we’ve managed to learn a lot and cobble together quite a diverse array of capabilities....contact your local space and get involved! Derek Jacoby is a PhD student at the University of Victoria....

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...versatile technique. Many thanks to our guest blogger Jacob Lazarus from Harvard. Jake received his MD and a...2014) :148–153. [accessed 2019 Feb 28]. Lazarus, Jacob E., et al. "A New Suite of Allelic Exchange Vectors...

  10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ... Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an RNA-guided ...Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.   ...

  12. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG... pegRNA BsmBI Yes (Cas9 H840A + MMLV RT) PuroTK Jacob Giehm Mikkelsen 174038 pU6-tevopreq1-GG-acceptor...

  13. Microbiology Resources

    Type
    Collection
    ...Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. Pseudomonas...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library 159391 Knockout Mouse Moffat 3rd 4 182 Mycobacterium tuberculosis CRISPRi Library (RLC12) 163954 ...Inhibition M. tuberculosis Rock NA Varies 96,700 Mycobacterium smegmatis CRISPRi Library (RLC11) 163955 Inhibition...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion proteins to...
Showing: 1 - 20 of 46 results