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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 38 results for: COB;

Showing: 1 - 20 of 38 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to their... “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA...efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune’s lab screened 11 other... 32:1141–1145 . https://doi.org/10.1038/nbt.3011 Cobb RE, Wang Y, Zhao H (2014) High-Efficiency Multiplex...2016) Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference...

  3. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if we could make... project can be a dangerous thing. Fortunately, Jacob was able to quickly explain that it was common knowledge...conformation that was not “poised for cleavage”. Jacob computationally added the non-template strand to...an effective teaching tool. You can read about Jacob’s initial reaction to this model on his blog. Or,...

  4. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...10.1093/genetics/iyae126.   Gene disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system...dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus, an increasingly common human pathogen...-based systems have been developed for other mycobacteria, but such systems don’t always work across species...for rapid and efficient genetic disruption in Mycobacterium abscessus. J Bacteriol., 206(3), e0033523. https...

  5. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Gray) and two MECP2 labs (Gail Mandel and Stuart Cobb) to create the Gene Therapy Consortium. Leveraging...10.1016/j.cell.2013.01.038 Guy J, Gan J, Selfridge J, Cobb S, Bird A (2007) Reversal of Neurological Defects...Gadalla KKE, Heindel C, Chen D, Zaric V, Bailey MES, Cobb SR, Gray SJ (2017) Improved MECP2 Gene Therapy Extends...Koerner MV, Gadalla KKE, Guy J, De Sousa D, Hector RD, Cobb SR, Bird A (2017) Radically truncated MeCP2 rescues...

  6. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...Understanding drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide (PZA...methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies ...

  7. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...The following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science...among our members we’ve managed to learn a lot and cobble together quite a diverse array of capabilities....contact your local space and get involved! Derek Jacoby is a PhD student at the University of Victoria....

  8. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...versatile technique. Many thanks to our guest blogger Jacob Lazarus from Harvard. Jake received his MD and a...2014) :148–153. [accessed 2019 Feb 28]. Lazarus, Jacob E., et al. "A New Suite of Allelic Exchange Vectors...

  9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an RNA-guided ...Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair pathways.   ...

  12. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Histidine bonds with immobilized metal ions (Nickel, Cobalt, Copper, etc.), allowing His fusion proteins to...

  15. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ... Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better design ...

  19. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128...
Showing: 1 - 20 of 38 results