Addgene

We narrowed to 21,474 results for: ESC;

Showing: 1541 - 1560 of 21474 results

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LifeAct-mGarnet

Plasmid

#104307

Purpose

codes for a fusion of fluorescent protein mGarnet and lifeact that targets F-actin in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

LifeAct-mGarnet

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEcgRNA

Plasmid

#166581

Purpose

CRISPR-Cas9-assisted genome editing in Escherichia coli

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-rsTagRFP

Plasmid

#31927

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

His

Expression

Bacterial

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

araB

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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miRFP670nano-pcDNA

Plasmid

#127443

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP

Plasmid

#80349

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Insert

smURFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Peredox

Plasmid

#73807

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-FL

Plasmid

#28291

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red

Plasmid

#48249

Purpose

Genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCMU-PDESr

Plasmid

#61206

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCX-H2B-KikGR

Plasmid

#98649

Purpose

Expresses a nuclear-localized photoconvertible green-to-red fluorescent protein

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Insert

KikGR

Use

Tags

H2B

Expression

Mammalian

Mutation

Promoter

CMV-IE enhancer chick beta actin promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only
Ecoli cpGFP

Plasmid

#215713

Purpose

Expresses cpGFP in Ecoli

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

circularly permuted GFP

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CDK4KTR

Plasmid

#126680

Purpose

Fluorescent reporter for CDK4/6 activity

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

RB cterm

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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Green Glifon600

Plasmid

#126207

Purpose

green fluorescent glucose sensor for mammalian cells

Depositor

Tetsuya Kitaguchi

Article

Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

Insert

Green Glifon600

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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H2B-mGarnet2

Plasmid

#104312

Purpose

codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells

Depositor

Ulrich Nienhaus

Article

Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

Insert

H2B-mGArnet2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23113

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-N1

Plasmid

#79987

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Has Service

Cloning Grade DNA

Insert

miRFP670

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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HRE-UnaG

Plasmid

#201710

Purpose

Hypoxia responsive promoter driving UnaG fluorescent proteins

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

HRE-mCMV

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Article

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Use

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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HRE-doUnaG

Plasmid

#201713

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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