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Showing: 1541 - 1560 of 2791 results

  1. pYPQAT46

    Plasmid
    #223418
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223418-plasmid-map-sequence-id-454166

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  2. pYPQAT47

    Plasmid
    #223419
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for monocot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-Mb2Cas12a-RVRR-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223419-plasmid-map-sequence-id-454168

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  3. pYPQAT48

    Plasmid
    #223420
    Purpose
    T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223420-plasmid-map-sequence-id-452225

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  4. pYFAC-ubi-Y-pyrG

    Plasmid
    #184497
    Purpose
    AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).
    Depositor
    Yit Heng Chooi
    Article
    Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010
    Type
    Empty backbone
    Use
    Synthetic Biology; Expression in aspergillus and …
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184497-plasmid-map-sequence-id-445302

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  10. pDOC-K-glmS-GFPrev

    Plasmid
    #158060
    Purpose
    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    Green Fluorescent Protein optimised for excitation with UV light
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    acpP
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158060-plasmid-map-sequence-id-309440

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  12. pHL 100

    Plasmid
    #52989
    Purpose
    pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    fhlA
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    −309 to +96 bp relative to start codon
    Promoter
    pLlac0-1 and pLtetO-1
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pHL 802

    Plasmid
    #52991
    Purpose
    pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    contains −81 to +36 bp relative to start codon
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pHL 1148

    Plasmid
    #53006
    Purpose
    pCon:OxyS, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pHL 1070

    Plasmid
    #53002
    Purpose
    pCon:DsrA, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pHL 1057

    Plasmid
    #53001
    Purpose
    pCon:RhyB, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    rpoS
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    containsn −150 to +30 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. CMV-Hylight

    Plasmid
    #193447
    Purpose
    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Hylight Biosensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193447-plasmid-map-sequence-id-378450

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Showing: 1541 - 1560 of 2791 results