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pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)

Plasmid

#55209

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xAsn, 10xHis, MPB, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-CGG-BsmBI

Plasmid

#222966

Purpose

0CGG with BsmBI site for inserting CGG repeats

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)

Use

CRISPR

Mutation

CGG repeats deleted from 5' UTR, BsmBI site …

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

Plasmid

#48322

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Thioredoxin

Expression

Bacterial

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV co-transformation cloning vector (13S-B)

Plasmid

#48324

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 prescission LIC cloning vector (HPKS)

Plasmid

#29720

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and Prescission cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

Plasmid

#48283

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV co-transformation cloning vector (13K-S)

Plasmid

#48321

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)

Plasmid

#30184

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector for hairpin mRNA (2U-T)

Plasmid

#29719

Depositor

Scott Gradia

Type

Empty backbone

Tags

MYFQSNA

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV co-transformation cloning vector (13K-C)

Plasmid

#48317

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and MBP

Expression

Bacterial

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Gamma crystallin TEV LIC cloning vector (2X-T)

Plasmid

#29714

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Gamma crystallin-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pET StrepII TEV co-transformation cloning vector (13S-R)

Plasmid

#48328

Depositor

Scott Gradia

Type

Empty backbone

Tags

StrepII

Expression

Bacterial

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pU6-Cj-sgRNA

Plasmid

#89753

Purpose

U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cj-SgRNA

Use

Sgrna expression under u6 promoter

Promoter

U6

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERN

Plasmid

#80474

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

Plasmid

#30185

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pME phluorin2-gpi

Plasmid

#73793

Purpose

ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

Depositor

David Raible

Article

Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

Insert

ratiometric pHluorin2-gpi

Use

Middle entry clone

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAG-ERP2

Plasmid

#80479

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pHREAC

Plasmid

#134908

Purpose

Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

double 35S

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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