167,150 results
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Plasmid#210470PurposePlasma membrane localizing mCherry using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mCherry (LCK Synthetic, Human, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCherryAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
FH-TET3-pEF
Plasmid#49446PurposeThis plasmid expresses full-length human TET3.DepositorInsertTet methylcytosine dioxygenase 3 (TET3 Human)
TagsFlag and HAExpressionMammalianPromoterEF-1alphaAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)
Plasmid#90086PurposeThis plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNADepositorInsertCas9
UseCRISPR and LentiviralTagsDestabilized Domain and FlagExpressionMammalianPromoterEFSAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX552
Plasmid#60958PurposepAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.DepositorInsertsU6_(SpaI)_sgRNA
Syn_EGFP-KASH
UseAAV, CRISPR, and Mouse TargetingExpressionMammalianPromoterSynapsin and U6Available SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMF440
Plasmid#62550PurposeBroad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.DepositorInsertmCherry
ExpressionBacterialPromotertrcAvailable SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG/GFP Asc
Plasmid#74285PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscIDepositorInsertRosa26 5-homology region
UseMouse TargetingAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL0284 (pQCascade_entry)
Plasmid#130635PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-HyPer7
Plasmid#136464PurposeMammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imagingDepositorInsertHyPer7
TagsLifeActExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralMutationD64V mutation within the integrase within PolAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR1-FusionRed
Plasmid#105678PurposeExpresses a soma-targeted GtACR1 in Cre-expressing neuronsDepositorHas ServiceAAV5InsertstGtACR1
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only