170,825 results
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Plasmid#79567Purposeexpression of CRY2PHR fused to phosphatase dead mutant of OCRLDepositorInsertOCRL (OCRL Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539 and phosphatase mutation, D523GPromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone
Plasmid#216155PurposeExpresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmScarlet with cryptic splice site and C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapERK)d1EGFP (KroxDs)
Plasmid#214912Purposefor PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activationDepositorInsertd1EGFP under the MapERK promoter
ExpressionMammalianPromoterMapkERK promoterAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIM1773-PRHIII-sfGFP-pNW33N
Plasmid#52217PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coliDepositorInsertPRHIII-sfGFP
ExpressionBacterialPromoterPRHIIIAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)
Plasmid#132926PurposepMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertLuc2P
UseLuciferase and Synthetic Biology; Pmvp gateway en…TagsPEST destabilization domainAvailable SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJS006
Plasmid#188329PurposePCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mNeonGreen::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Myc-Rab5A S34N
Plasmid#28044DepositorAvailable SinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only