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pIG-749_HA-GD2-28z_CAR_TFAP4

Plasmid

#207490

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-852_HA-GD2-28z_TFAP4-HA

Plasmid

#207502

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

Plasmid

#211819

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and blasticidin resistance gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter and miniU6 promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Expression

Mammalian

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

Plasmid

#211815

Purpose

lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9, tracrRNA and puromycin selection gene

Use

Lentiviral

Expression

Mammalian

Promoter

U1a promoter, U6 promoter, and hPGK promoter

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(C)

Plasmid

#206142

Purpose

Expresses the rs27895-C allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-PBX4-P2A-mCherry

Plasmid

#203864

Purpose

Tet-inducible lentiviral plasmid expressing PBX4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

PBX4 (PBX4 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgCtrl#2

Plasmid

#174143

Purpose

Lentiviral vector expressing a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-Lyn-AktAR2-D

Plasmid

#125198

Purpose

SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

Lyn-AktAR2-D

Use

Transposon

Tags

Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

Expression

Mammalian

Mutation

FoxO1 Thr-24 changed to Val rendering this inacti…

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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