We narrowed to 875 results for: seph

1. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

   Plasmid

   #133796

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1 (truncated C-terminal domain only)
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   C-terminal domain only

   Promoter

   CMV and T7

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)

   Plasmid

   #133798

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1(T114A/F115A)
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   T114A/F115A

   Promoter

   CMV and T7

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

   Plasmid

   #133791

   Purpose

   U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St1Cas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V1 sgRNA architecture from Carter et al. biorxiv …

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

   Plasmid

   #133792

   Purpose

   U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St3Cas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Muller et al. Molecular T…

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(R47A)

   Plasmid

   #112722

   Purpose

   Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(R47K)

   Plasmid

   #112723

   Purpose

   Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R47K)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R47K

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_6HT-TBP6.7(Q48A)

   Plasmid

   #112724

   Purpose

   Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_6HT-TBP6.7(T50A)

   Plasmid

   #112727

   Purpose

   Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(T50A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   T50A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_6HT-TBP6.7(R52A)

   Plasmid

   #112729

   Purpose

   52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R52A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R52A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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