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We narrowed to 5,023 results for: cre encoding plasmid

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Showing: 3121 - 3160 of 5023 results

  4. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available Since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

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  5. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid
    #157716
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157716-plasmid-map-sequence-id-307912

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  6. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid
    #157699
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157699-plasmid-map-sequence-id-307863

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  7. SMT000

    Plasmid
    #216849
    Purpose
    Plasmid that encodes a sfGFP gene under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramphenicol resistant.
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Superfolder Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    S30R, Y39N, N105T, Y145F, I171V, and A206V
    Promoter
    T7
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    216849-plasmid-map-sequence-id-433256

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  8. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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  9. pBAD-MTX-u51_Snifit

    Plasmid
    #119046
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u51_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    51TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119046-plasmid-map-sequence-id-229503

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  10. pBAD-MTX-u23_Snifit

    Plasmid
    #119044
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u23_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    23TAG R44L H45Q
    Promoter
    pBAD
    Available Since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119044-plasmid-map-sequence-id-229496

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  11. pBACH2-donor

    Plasmid
    #176357
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    BACH2 (BACH2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176357-plasmid-map-sequence-id-347870

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  13. pSOX2-donor

    Plasmid
    #176364
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176364-plasmid-map-sequence-id-347872

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  14. pID3-donor

    Plasmid
    #176342
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ID3 (ID3 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176342-plasmid-map-sequence-id-347890

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  15. pCDX4-donor

    Plasmid
    #176343
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    CDX4 (CDX4 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176343-plasmid-map-sequence-id-347886

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  16. pJAZF1-donor

    Plasmid
    #176352
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    JAZF1 (JAZF1 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176352-plasmid-map-sequence-id-347876

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  17. pTP63-donor

    Plasmid
    #132477
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132477-plasmid-map-sequence-id-273771

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  18. pE4F1.1.0-gDNA

    Plasmid
    #132451
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    E4F1 (E4F1 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132451-plasmid-map-sequence-id-266819

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  24. EBOV 4cis

    Plasmid
    #249332
    Purpose
    Integration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)
    Depositor
    Amy Kistler
    Insert
    EBOV NP-P2A-VP35-P2A-VP30-P2A-L protein
    Use
    Piggybac donor vector
    Expression
    Bacterial and Mammalian
    Mutation
    the EBOV NP, VP35, and VP30 ORF sequences within …
    Promoter
    CAG
    Available Since
    Jan. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    249332-plasmid-map-sequence-id-497977

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  33. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

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  34. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126758
    Purpose
    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126758-plasmid-map-sequence-id-412365

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  35. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126759
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126759-plasmid-map-sequence-id-412375

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  36. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126754
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126754-plasmid-map-sequence-id-410972

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  37. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126756
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126756-plasmid-map-sequence-id-410579

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  40. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

    Plasmid
    #126755
    Purpose
    Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126755-plasmid-map-sequence-id-410971

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Showing: 3121 - 3160 of 5023 results