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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,300 results for: Pet2

Showing: 3901 - 3950 of 4300 results

  1. SpyTag003-OspC

    Plasmid
    #159998
    Purpose
    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells
    Depositor
    Mark Howarth
    Article
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Insert
    SpyTag003-OspC (20-209) C129S
    Use
    Tags
    His6-tag and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    C129S mutation improves expression level.
    Promoter
    T7
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159998-plasmid-map-sequence-id-316165

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  21. GRLF1

    Plasmid
    #36195
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains the Ras homolog domain only (amino acid …
    Promoter
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Use
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available Since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  23. NmMetQ N238A

    Plasmid
    #129648
    Purpose
    Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence
    Depositor
    Douglas Rees
    Article
    Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p
    Insert
    metQ Gene
    Use
    Tags
    10x Histidine Tag, Enterokinase, and pelB signal …
    Expression
    Bacterial
    Mutation
    Changed Asparagine 238 to Alanine
    Promoter
    T7 Promoter
    Available Since
    Sept. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    129648-plasmid-map-sequence-id-254340

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  24. pLyGo-Ec-6

    Plasmid
    #163132
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163132-plasmid-map-sequence-id-338662

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  33. L3MBTL3 (3UT1)

    Plasmid
    #36901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3 (L3MBTL3 Human)
    Use
    Tags
    His tag and Streptavidin-Binding Peptide (SBP)-Ta…
    Expression
    Bacterial
    Mutation
    contains amino acid residues 228-551 only
    Promoter
    Available Since
    June 21, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Use
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  45. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif3A (Kif3a Mouse)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229852-plasmid-map-sequence-id-460709

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  49. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-546 (USP7 Human)
    Use
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229001-plasmid-map-sequence-id-458328

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Showing: 3901 - 3950 of 4300 results