-
Plasmid#19076DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationD34APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 E18A
Plasmid#19077DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationE18APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 E18A/D22A
Plasmid#19078DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationE18A/D22APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 D21A/D22A
Plasmid#19079DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationD21A/D22APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 F199A
Plasmid#19080DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationF199APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 E231A
Plasmid#19081DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationE231APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 R42A
Plasmid#19083DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationR42APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098 R80A
Plasmid#19084DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationR80APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098(1-129)
Plasmid#19086DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098(130-242)
Plasmid#19087DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tag and N tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098(243-339)
Plasmid#19088DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098(1-242)
Plasmid#19089DepositorInsertCell division protein pelota related protein (TA_RS05685 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Ta1098(130-339)
Plasmid#19090DepositorInsertCell division protein pelota related protein (DOM34 Thermoplasma acidophilum)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
AF0837(128-241)
Plasmid#19093DepositorInsertCell division protein pelota (DOM34 Archaeoglobus fulgidus)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
AF0837(242-344)
Plasmid#19094DepositorInsertCell division protein pelota (DOM34 Archaeoglobus fulgidus)
UseTagsC tagExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dom34 E23K/F218A
Plasmid#19106DepositorInsertProtein DOM34 (DOM34 Budding Yeast)
UseTagsC tagExpressionBacterialMutationE23K/F218APromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCeSR beta deltaN
Plasmid#13728DepositorInsertSignal recognition particle receptor β subunit (R186.3 Nematode)
UseTags6xHisExpressionBacterialMutationexpresses residues 38-240 of C. elegans SRβ with …PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pScSR beta deltaN Flag
Plasmid#13729DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHis and FlagExpressionBacterialMutationexpresses residues 31-244 of S. cerevisiae SRβ wi…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
His6-pAG-RTase
Plasmid#214932PurposeExpress fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systemsDepositorInsertfusion protein of protein A/G and MMLV reverse transcriptase (25-497)
UseTagsHis6ExpressionBacterialMutationMMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…PromoterT7 promoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF1
Plasmid#228775PurposeProtein expression plasmid for YTH domain of YTHDF1 in E.Coli.DepositorInsertYTH domain of YTHDF1 (YTHDF1 Human)
UseTags6*HisExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKD068
Plasmid#136471PurposeSSB-GFP IDL fusion expression plasmidDepositorInsertSSB-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC001 - huLshC2C2-MBP for bacterial expression
Plasmid#79150PurposeExpresses human codon-optimized LshC2c2 for purification in E. coliDepositorInsertC2c2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
hCD12
Plasmid#225541PurposeExpresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.DepositorInsertHuman Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
UseTagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET 28 full-length human PARP1
Plasmid#192635PurposeExpresses human PARP1 with an N-terminal His-tagDepositorInsertHuman PARP1 (PARP1 Human)
UseTagshistidine-tagExpressionBacterialMutationPolymorphism A762PromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
WT USP7 catalytic
Plasmid#227201PurposeExpresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationaa 207-560PromoterT7-promoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
UseTags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C
Plasmid#184410Purposebacterial expression of TTRDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85CPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
JF001A
Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB8 (murine) HIS-tag pET
Plasmid#8540DepositorInsertHOXB8 (Hoxb8 Mouse)
UseTagsHis and T7ExpressionBacterialMutationstarts at amino acid #2 (Ser)PromoterAvailable sinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXA7 (murine) His-tag pET
Plasmid#8491DepositorInsertHOXA7 (Hoxa7 Mouse)
UseTagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATG; makes protein by …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-(pos36)GFP-9xGGS-Cre-6xHis
Plasmid#62938PurposeExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cellsDepositorInsert(ps36)GFP-9xGGS-Cre
UseTags(pos36)GFP and 6xHis tagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
CBP TAZ1(340-439)
Plasmid#173760Purposebacterial expression of mouse CBP TAZ1 domainDepositorInsertCBP TAZ1 (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) HA-tag pET
Plasmid#8489DepositorInsertHOXA4 (Hoxa4 Mouse)
UseTagsHA and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
-
HOXA10 (human) HIS-tag pET
Plasmid#8516DepositorInsertHOXA10 (HOXA10 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-kan
Plasmid#108703PurposeExpression of an His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQLN2_450-624W
Plasmid#176852PurposeExpresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cellsDepositorInsertUBQLN2_450C_W (UBQLN2 Human)
UseTagsExpressionBacterialMutationdeletion of residues 1-449 and addition of C-term…PromoterT7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80D
Plasmid#184743Purposebacterial expression of human TTR mutantsDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85C, K80DPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXB2 (human) HIS-tag pET
Plasmid#8522DepositorInsertHOXB2 (HOXB2 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at AA #2; makes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pZJQIBEBT003
Plasmid#140053PurposeThe pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.DepositorInserteMutS-L157C-G233C-CBM-EGFP
UseTagsHis-TagExpressionBacterialMutationL157C, G233CPromoterT7Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXB7 (human) HIS-tag pET
Plasmid#8538DepositorInsertHOXB7 (HOXB7 Human)
UseTagsHis and T7ExpressionBacterialMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHelA12
Plasmid#176493PurposeExpresses the N-terminal His6-tagged SARS-CoV helicase in E coli.DepositorInsertSARS-CoV NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationV570IPromoterT7Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-CH2 (1192-1318)
Plasmid#99338PurposeBacterial expression of mouse CBP CH2 domainDepositorInsertCBP CH2 (1192-1318) (Crebbp Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) HIS-tag pET
Plasmid#8553DepositorInsertHOXD12 (Hoxd12 Mouse)
UseTagsHis and T7ExpressionBacterialMutationFusion sequence is: ggatccATGPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-RABIF
Plasmid#135556PurposeExpress human RABIF in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertRABIF (RABIF Human)
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) pET
Plasmid#8488DepositorInsertHOXA4 (Hoxa4 Mouse)
UseTagsHis and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK597
Plasmid#71121PurposeProduces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only